产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
人类CCL2/MCP1 EZ套酶联免疫吸附试剂盒(DIY抗体配对)
目录 :
EZ0441
规格 :
For 5 plates, 96 wells each
价格 :
255美元
图像
图像 1 :
武汉博士德生物工程有限公司 EZ0441 图像 1
Human CCL2/MCP1 EZ Set ELISA Kit standard curve
产品信息
SKU号 :
EZ0441
状态 :
Enabled
名称 :
人类CCL2/MCP1 EZ套酶联免疫吸附试剂盒(DIY抗体配对)
目录名称 :
Picokine ELISA Kits, EZ Set ELISA Kits (Ab Pairs)
基因名称 :
CCL2
价格 :
255美元
试验范围 :
15.6pg/毫升1000pg/毫升
componentsneeded :
Components Included In The EZ Set Accessory Kit Antibody diluent buffer ABC diluent buffer Sample diluent buffer TMB TMB stop solution 5x 96 well plates, plate sealers . *The EZ Set Accessory Kit provides an easy way to get all the reagents you need for performing an ELISA assay. The components included in the EZ Set Accessory Kit can also be substituted with products other companies if it is of convenience to you. Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects CCL2/MCP1in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
short description :
A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects CCL2/MCP1in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
description1 :
Product Features: . Your ideal low cost ELISA solution. EZ Set ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies. . Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.
规格 :
For 5 plates, 96 wells each
UniProt数据库编号 :
P13500
宿主 :
No
免疫原 :
Expression system for standard: E.coli; Immunogen sequence: Q24-T99
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is cross-reactivity with human Eotaxin, MCP-3<1%.
敏感性 :
<1pg/ml
kit components :
Catalog number Description Quantity Buffers EZ0441-CA Rabbit anti- human CCL2/MCP1 polyclonal antibody(Capture Antibody) 500 uL, 0.4mg/mL(recommended dilution 1:100) 0.5% Na2HPO4, 0.02% Sodium azide and 50% Glycerol EZ0441-DA Biotinylated goat anti- human CCL2/MCP1 polyclonal antibody(Detection Antibody) 500 uL, 5ug/mL(recommended dilution 1:100) 0.02% Sodium azide, 0.5%BSA, 0.2%Tris, 1%NaCl and 30% Glycerol EZ0441-ST Lyophilized recombinant human CCL2/MCP1 standard 10ng/tubex2 AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 500ul(recommended dilution 1:100)
样品类型 :
cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.
应用 :
酶联免疫吸附测定
反应物种 :
人类
图像标记 :
人类CCL2/MCP1 EZ套酶联免疫吸附试剂盒标准曲线
anticoagulant :
肝素, 乙二胺四乙酸, 柠檬酸盐
assay procedure step 1 :
1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升, 15.6pg/毫升
背景 :
Monocyte chemoattractant protein-1(MCP-1), a member of the chemokine(chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.
同义词 :
C-C motif chemokine 2;HC11;Monocyte chemoattractant protein 1;Monocyte chemotactic and activating factor;MCAF;Monocyte chemotactic protein 1;MCP-1;Monocyte secretory protein JE;Small-inducible cytokine A2;CCL2;MCP1, SCYA2
基因全称 :
C-C motif chemokine 2
protein function :
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
subcellular localization :
Secreted.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 31 159 477 30 148 481 Standard deviation 1.55 8.74 32.91 1.98 9.76 37.51 CV(%) 5% 5.5% 6.9% 6.6% 6.6% 7.8%
样品数据 :
Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.100 0.206 0.426 0.826 1.271 1.762 2.087
tmb incubation time :
15-20min
更新 :
5/23/19 3:37
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits