ELISA/assay

Human Tryptase/TPSAB1,B2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

EZ0898

For 5 plates, 96 wells each

270美元

Human Tryptase/TPSAB1,B2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

For 5 plates, 96 wells each

270美元

Human Tryptase/TPSAB1,B2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human TPSB2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15 pg/ml. Product Features: Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

人类

酶联免疫吸附测定

TPSB2

P20231

Expression system for standard: NS0; Immunogen sequence: M1-V275

There is no detectable cross-reactivity with other relevant proteins.

156 pg/ml - 10,000 pg/ml

<15 pg/ml

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Tryptase beta-2

Tryptase beta-2;Tryptase-2;3.4.21.59;Tryptase II;TPSB2;TPS2;

Tryptase alpha/beta-1/beta-2

30515分子量

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.

Secreted. Released from the secretory granules upon mast cell activation.

TPSAB1: Isoform 1 and isoform 2 are expressed in lung,stomach, spleen, heart and skin; in these tissues, isoform 1 ispredominant. Isoform 2 is expressed in aorta, spleen, and breasttumor, with highest levels in the endothelial cells of some bloodvessels surrounding the aorta, as well as those surrounding thetumor and low levels, if any, in mast cells (at protein level).

Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

Cell Biology, Immunology, Innate Immunity, Interspecies Interaction, Microbiology, Proteolysis / Ubiquitin, Proteolytic Enzymes, Secreted Molecules