产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-human CD14 Monoclonal Antibody FITC Conjugated, Flow Validated
目录 :
FC00137-异硫氰酸荧光素
规格 :
100试验, 200试验
价格 :
164美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
异硫氰酸荧光素
克隆名称 :
26ic
反应物种 :
人类
应用 :
流式细胞仪
图像
图像 1 :
武汉博士德生物工程有限公司 FC00137-FITC 图像 1
Monocytes gated PBMCs stained with FITC conjugated anti-human CD14 (clone 26iC) (red histogram). Monocytes gated PBMCs stained with anti-human clone IgG2b FITC (blue histogram).
产品信息
SKU号 :
FC00137-异硫氰酸荧光素
产品名称 :
Anti-human CD14 Monoclonal Antibody FITC Conjugated, Flow Validated
价格 :
164美元
规格 :
100试验, 200试验
克隆性 :
单克隆
克隆编号 :
26ic
宿主 :
小鼠
共轭标签 :
异硫氰酸荧光素
反应物种 :
人类
应用(s) :
流式细胞仪
应用细节 :
User need to optimize the dilution ratio for this antibody
描述 :
Boster Bio Anti-human CD14 Monoclonal Antibody FITC Conjugated, Flow Validated catalog # FC00137-FITC. Tested in Flow Cytometry applications. This antibody reacts with Human. Introduction: Clone 26ic reacts with a nonfunctional domain of human CD14, a 53-55 kDa glycosylphosphatidylinositol (GPI)-anchored and single chain glycoprotein expressed at high levels on monocytes. Additionally, CD14 antibody reacts with interfollicular macrophages, reticular dendritic cells and some Langerhans cells. The binding of CD14 antibody does not inhibit CD14 mediated activities, and is useful for detecting CD14 expression by immunofluorescence and or immunocytochemical methods. This antibody is routinely tested by flow cytometric analysis. Flow cytometry and other applications were tested during antibody development by Boster or are reported in the literature. Application Information: Each lot of this antibody has been pre-titrated and tested by flow cytometric analysis of human PBMCs such that 5µl of this product is sufficient for staining of 1 million cells in a 100µl staining volume or 100µl of whole blood. It is recommended that antibody reactivity be empirically titrated for optimal performance in the application of interest.
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
CD14
UniProt数据库编号 :
P08571
形式 :
液体
Contents :
PBS, pH7.2, 0.09% NaN3和0.2%(w/v)BSA
纯化 :
蛋白质A纯化
抗体亚型 :
IgG2b, k
储存 :
4°C and protect from prolonged exposure to light. Do not freeze.
基因全称 :
单核细胞 分化类抗原CD14
同义词 :
Monocyte differentiation antigen CD14 ; Myeloid cell-specific leucine-rich glycoprotein; CD14; Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form; CD14;
蛋白名 :
单核细胞 分化类抗原CD14
分子量 :
40076分子量
Protein Function :
Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL (-)) and mediates the cytokine release induced by LDL (-) (PubMed:23880187).
Subcellular Localization :
Cell membrane ; Lipid- anchor, GPI-anchor. Secreted. Membrane raft. Golgi apparatus. Secreted forms may arise by cleavage of the GPI anchor.
组织特异性 :
Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
背景 :
CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
研究种类 :
Cell Type Markers, Hematopoietic Progenitors, Immunology, Innate Immunity, Mesenchymal Stem Cells, Myeloid, Stem Cells, Tlr Signaling
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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