抗体

Anti-human HLA-DR Monoclonal Antibody Unconjugated, Flow Validated

FC00568

100µg, 500µg

55美元

单克隆

小鼠

nonconjugated

L243

Enabled

Anti-human HLA-DR Monoclonal Antibody Unconjugated, Flow Validated

一抗, 单克隆抗体, 流式细胞仪抗体

HLA-DRA

55美元

100µg/$55 500µg/$225

单克隆

L243

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

No

PBS, pH7.2, 0.09% NaN3

Monoclonal clone# G2 antibody for HLA DR/HLA-DRA detection. Host: Mouse.Size: 100µg, 500µg. Tested applications: Flow Cytometry. Reactive species: Human. HLA DR/HLA-DRA information: Molecular Weight: 28607 MW; Subcellular Localization: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Endosome membrane; Single- pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.

Mouse Monoclonal Anti-human HLA-DR Unconjugated, designed for Flow Cytometry and validated by Flow Cytometry using Human cells.

Introduction . The L243 antibody reacts with human HLA-DR antigen which is expressed on B lymphocytes, monocytes, macrophages, activated T lymphocytes, activated natural killer (NK) lymphocytes, and human progenitor cells. HLADR is also present on thymic epithelium, B lymphocyte dependent areas of spleen and lymph node, and B cell lymphomas. . This antibody is routinely tested by flow cytometric analysis. Flow cytometry and other applications were tested during antibody development by CapricoBio or are reported in the literature. Application Information . Each lot of this antibody has been quality control tested by flow cytometric analysis of human PBMCs. For flow cytometric staining, the recommended use of this antibody is 0.5ug per 1x106 cells in 100ul of staining volume. It is strongly suggested that the antibody reactivity be empirically titrated for optimal performance in the application of interest.

100µg, 500µg

P01903

小鼠

人类B淋巴细胞

Liquid

蛋白质A

2-8 C

IgG2a, k

5ul per sample

User need to optimize the dilution ratio for this antibody

流式细胞仪

人类

Lymphocytes gated PBMCs stained with purified anti-human HLA-DR (clone L243) followed by Goat anti-mouse IgG (H+L) Alexa Fluor 488 (red histogram). Lymphocytes gated PBMCs stained with only Goat anti-mouse IgG (H+L) Alexa Fluor 488 (Blue histogram).

HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD alpha (heavy) chain and a 27 kD beta (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.

HLA组织相容性抗原II类, DR甲链;MHC II类抗原DRA;HLA-DRA;HLA-DRA1;

HLA组织相容性抗原II类, DR甲链

28607分子量

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Endosome membrane; Single- pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.

HLA组织相容性抗原II类, DR甲链/DRB1-1乙链

Belongs to the MHC class II family.

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