抗β微管蛋白兔单克隆抗体, HRP共轭标签 | 武汉博士德生物工程有限公司 H01857 产品信息

产品简要

公司名称 :

武汉博士德生物工程有限公司

产品类型 :

抗体

产品名称 :

抗β微管蛋白兔单克隆抗体, HRP共轭标签

H01857

规格 :

100微升

价格 :

315美元

克隆性 :

单克隆

宿主 :

domestic rabbit

共轭标签 :

未共轭

克隆名称 :

DF-20

反应物种 :

人类, 小鼠, 大鼠

应用 :

免疫印迹

更多信息或购买 :

武汉博士德生物工程有限公司产品网页

图像

图像 1 :

Western blot analysis of beta Tubulin expression in (1) Jurkat cell lysate, (2) Human kidney lysate, (3) 3T3 cell lysate, (4) Mouse brain lysate, (5) C6 cell lysate, (6) Rat heart lysate (H01857). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB3 monoclonal antibody (Catalog # H01857) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBB3

产品信息

SKU号 :

H01857

产品名称 :

抗β微管蛋白兔单克隆抗体, HRP共轭标签

价格 :

315美元

规格 :

100微升

克隆性 :

单克隆

克隆编号 :

DF-20

宿主 :

兔

反应物种 :

人类, 小鼠, 大鼠

应用(s) :

免疫印迹

应用细节 :

免疫印迹1:5000-1:20000

描述 :

Boster Bio Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated catalog # H01857. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

浓度 :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

基因名称 :

Ⅲ型β微管蛋白

UniProt数据库编号 :

Q13509

免疫原 :

A synthesized peptide derived from human beta Tubulin

形式 :

液体

Contents :

兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA

纯化 :

亲和色谱

储存 :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

基因全称 :

微管蛋白乙3链

同义词 :

微管蛋白乙3链;微管蛋白乙4链;微管蛋白乙III;Ⅲ型β微管蛋白;TUBB4;

分子量 :

50433分子量

Protein Function :

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

Subcellular Localization :

细胞质, 细胞骨架

组织特异性 :

Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.

研究种类 :

Cell Adhesion Proteins, Cell Type Markers, Cytoskeleton, Cytoskeleton / Ecm, Microtubules, Neuroscience, Signal Transduction, Tubulin

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公司信息