产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗p53单克隆抗体(PAb122)
目录 :
M00001-4
规格 :
200微克
价格 :
397美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
Pab122
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
图像
图像 1 :
武汉博士德生物工程有限公司 M00001-4 图像 1
Figure 3. IHC analysis of Tp53 using anti-Tp53 antibody (M00001-4). Tp53 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Tp53 Antibody (M00001-4) overnight at 4 C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
图像 2 :
武汉博士德生物工程有限公司 M00001-4 图像 2
Figure 2. IHC analysis of Tp53 using anti-Tp53 antibody (M00001-4). Tp53 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Tp53 Antibody (M00001-4) overnight at 4 C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
图像 3 :
武汉博士德生物工程有限公司 M00001-4 图像 3
Figure 1. Western blot analysis of Tp53 using anti-Tp53 antibody (M00001-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tp53 antigen affinity purified polyclonal antibody (Catalog # M00001-4) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Tp53.
产品信息
SKU号 :
M00001-4
状态 :
Enabled
名称 :
抗p53单克隆抗体(PAb122)
目录名称 :
一抗, 单克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
Tp53
价格 :
397美元
price various sizes :
200ug / $397 200ug+Free HRP Secondary BA1050 / $397 200ug+Free Biotin Secondary BA1001 / $397
克隆性 :
单克隆
克隆编号 :
pAb122
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
描述 :
Mouse Monoclonal pAb122 antibody for Tp53 detection. Tested positive for Flow Cytometry, IF, IHC, IP, WB in Human, Mouse, Rat
short description :
Mouse Monoclonal pAb122 antibody for Tp53 detection. Tested positive for Flow Cytometry, IF, IHC, IP, WB in Human, Mouse, Rat
规格 :
200微克
UniProt数据库编号 :
P02340
宿主 :
小鼠
免疫原 :
SV40 transformed 3T3 cells.
形式 :
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
纯化 :
蛋白质G亲和纯化
储存 :
-20 C
应用细节 :
Western Blot (1:250 colorimetric, 1:250 ECL) . Immunohistochemistry (1:20 - 1:100) . Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
应用 :
免疫沉淀, IF, 免疫组化, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Figure 3. IHC analysis of Tp53 using anti-Tp53 antibody (M00001-4). Tp53 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Tp53 Antibody (M00001-4) overnight at 4 C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. Figure 2. IHC analysis of Tp53 using anti-Tp53 antibody (M00001-4). Tp53 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Tp53 Antibody (M00001-4) overnight at 4 C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. Figure 1. Western blot analysis of Tp53 using anti-Tp53 antibody (M00001-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tp53 antigen affinity purified polyclonal antibody (Catalog # M00001-4) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Tp53.
研究种类 :
Apoptosis, Cell Biology, Intracellular, P53 Pathway
更新 :
2/6/19 22:57
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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