产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-beta Catenin Picoband Antibody (monoclonal, 1F6)
目录 :
M00004-2
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
1F6
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
图像
图像 1 :
武汉博士德生物工程有限公司 M00004-2 图像 1
Figure 1. Western blot analysis of beta Catenin using anti-beta Catenin antibody (M00004-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, . Lane 2: human COLO-320 whole cell lysates, . Lane 3: human HepG2 whole cell lysates, . Lane 4: human MCF-7 whole cell lysates, . Lane 5: human SW620 whole cell lysates, . Lane 6: human 22RV1 whole cell lysates, . Lane 7: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-beta Catenin antigen affinity purified monoclonal antibody (Catalog # M00004-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
图像 2 :
武汉博士德生物工程有限公司 M00004-2 图像 2
Figure 2. IHC analysis of beta Catenin using anti-beta Catenin antibody (M00004-2). beta Catenin was detected in paraffin-embedded section of human mammary cancer . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-beta Catenin Antibody (M00004-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
图像 3 :
武汉博士德生物工程有限公司 M00004-2 图像 3
Figure 3. IHC analysis of beta Catenin using anti-beta Catenin antibody (M00004-2). beta Catenin was detected in paraffin-embedded section of human mammary cancer . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-beta Catenin Antibody (M00004-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
产品信息
SKU号 :
M00004-2
状态 :
Enabled
名称 :
Anti-beta Catenin Picoband Antibody (monoclonal, 1F6)
目录名称 :
一抗, 单克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
CTNNB1
价格 :
280美元
price various sizes :
Unconjugated / $280 APC / $330 APC-Cy7 / $330 FITC / $330 PE / $330 PE-Cy5 / $330 PE-Cy7 / $330 100ug / $280 100ug+Free HRP Secondary BA1050 / $280 100ug+Free Biotin Secondary BA1001 / $280
克隆性 :
单克隆
克隆编号 :
1F6
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Mouse IgG monoclonal antibody for beta Catenin detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.
short description :
Mouse IgG monoclonal antibody for beta Catenin detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.
规格 :
100 ug/vial
UniProt数据库编号 :
P35222
宿主 :
小鼠
免疫原 :
E. coli-derived human beta Catenin recombinant protein (Position: A2-K233).
形式 :
冻干
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins.
抗体亚型 :
IgG1
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
免疫印迹, 0.1 0.5微克/毫升。 免疫组化(石蜡包埋切片), 0.5-1微克/毫升。 免疫细胞化学/免疫荧光, 2微克/毫升。 流式细胞仪, 1-3微克/1x10 6细胞
应用 :
流式细胞仪, IF, 免疫组化-P, 免疫细胞化学, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Figure 1. Western blot analysis of beta Catenin using anti-beta Catenin antibody (M00004-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, . Lane 2: human COLO-320 whole cell lysates, . Lane 3: human HepG2 whole cell lysates, . Lane 4: human MCF-7 whole cell lysates, . Lane 5: human SW620 whole cell lysates, . Lane 6: human 22RV1 whole cell lysates, . Lane 7: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-beta Catenin antigen affinity purified monoclonal antibody (Catalog # M00004-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. Figure 2. IHC analysis of beta Catenin using anti-beta Catenin antibody (M00004-2). beta Catenin was detected in paraffin-embedded section of human mammary cancer . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-beta Catenin Antibody (M00004-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. Figure 3. IHC analysis of beta Catenin using anti-beta Catenin antibody (M00004-2). beta Catenin was detected in paraffin-embedded section of human mammary cancer . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-beta Catenin Antibody (M00004-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. Figure 4. IHC analysis of beta Catenin using anti-beta Catenin antibody (M00004-2). beta Catenin was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-beta Catenin Antibody (M00004-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. Figure 5. Flow Cytometry analysis of SiHa cells using anti-beta Catenin antibody (M00004-2). Overlay histogram showing SiHa cells stained with M00004-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-beta Catenin Antibody (M00004-2,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control. Figure 6. IF analysis of beta Catenin using anti- beta Catenin antibody (M00004-2) . beta Catenin was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL mouse anti- beta Catenin Antibody (M00004-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
背景 :
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
研究种类 :
Actin Etc, Atherosclerosis, Cadherins, Cancer, Cardiovascular, Cell Adhesion, Cytoplasmic, Cytoskeleton, Cytoskeleton / Ecm, Invasion/Microenvironment, Microfilaments, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction, Signaling Pathways, Stem Cells, Vascular Inflammation
同义词 :
Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; OK/SW-cl.35, PRO2286
基因全称 :
catenin beta 1
protein function :
Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity).
subcellular localization :
Cytoplasm. Nucleus
组织特异性 :
Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level).
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti- Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
更新 :
6/3/19 9:03
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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