产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗v-erb-b2成红细胞白血病病毒致瘤基因2(HER2)单克隆抗体
目录 :
M00010-1
规格 :
100微克
价格 :
299美元
克隆性 :
单克隆
宿主 :
共轭标签 :
nonconjugated
克隆名称 :
BCI-5
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
图像
图像 1 :
武汉博士德生物工程有限公司 M00010-1 图像 1
Figure 1. Western blot analysis of ERBB2 using anti-ERBB2 antibody in SKBR-3 cell lysate. (M00010-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB2 antigen affinity purified polyclonal antibody (Catalog # M00010-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ERBB2.
图像 2 :
武汉博士德生物工程有限公司 M00010-1 图像 2
Immunofluorescent analysis of SKBR cells, using ErbB2 (HER2) Antibody.
图像 3 :
武汉博士德生物工程有限公司 M00010-1 图像 3
Figure 2. IHC analysis of ERBB2 using anti-ERBB2 antibody in paraffin-embedded human breast carcinoma (M00010-1). ERBB2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ERBB2 Antibody (M00010-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
M00010-1
状态 :
Enabled
名称 :
抗v-erb-b2成红细胞白血病病毒致瘤基因2(HER2)单克隆抗体
目录名称 :
一抗, 单克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
v-erb-b2成红细胞白血病病毒致瘤基因2
价格 :
299美元
price various sizes :
100ug / $299 100ug+Free HRP Secondary BA1054 / $299 100ug+Free Biotin Secondary BA1003 / $299 30ug sample size / $99
克隆性 :
单克隆
克隆编号 :
BCI-5
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油
描述 :
Rabbit IgG Monoclonal antibody for ErbB2 detection. Tested positive for WB, IHC-P, ICC, IF, IP, Flow Cytometry in Human
short description :
Rabbit IgG Monoclonal antibody for ErbB2 detection. Tested positive for WB, IHC-P, ICC, IF, IP, Flow Cytometry in Human
规格 :
100微克
sample size available :
30ul for $99, contact us for details
UniProt数据库编号 :
P04626
宿主 :
免疫原 :
A synthesized peptide derived from human ErbB2 (HER2).
形式 :
Liquid
纯化 :
亲和色谱
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
应用细节 :
免疫印迹1:500~1:2000。 免疫组化-P 1:50~1:200。 免疫细胞化学/IF 1:50~1:200。 免疫沉淀1:50。 FCM 1:50
应用 :
免疫沉淀, IF, 免疫组化, 免疫印迹
反应物种 :
人类
图像标记 :
Figure 1. Western blot analysis of ERBB2 using anti-ERBB2 antibody in SKBR-3 cell lysate. (M00010-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB2 antigen affinity purified polyclonal antibody (Catalog # M00010-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ERBB2. Immunofluorescent analysis of SKBR cells, using ErbB2 (HER2) Antibody. Figure 2. IHC analysis of ERBB2 using anti-ERBB2 antibody in paraffin-embedded human breast carcinoma (M00010-1). ERBB2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ERBB2 Antibody (M00010-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
研究种类 :
Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens, Tyrosine Kinases
更新 :
4/22/19 1:33
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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