产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗AKT1单克隆抗体
目录 :
M00024-1
规格 :
100微克
价格 :
299美元
克隆性 :
单克隆
宿主 :
共轭标签 :
nonconjugated
克隆名称 :
EBG-1
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
图像
图像 1 :
武汉博士德生物工程有限公司 M00024-1 图像 1
Immunofluorescent analysis of Hela cells, using AKT1 Antibody.
图像 2 :
武汉博士德生物工程有限公司 M00024-1 图像 2
Figure 2. IHC analysis of AKT1 using anti-AKT1 antibody (M00024-1). AKT1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AKT1 Antibody (M00024-1) overnight at 4 C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
武汉博士德生物工程有限公司 M00024-1 图像 3
Figure 1. Western blot analysis of AKT1 using anti-AKT1 antibody (M00024-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 antigen affinity purified polyclonal antibody (Catalog # M00024-1) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for AKT1.
产品信息
SKU号 :
M00024-1
状态 :
Enabled
名称 :
抗AKT1单克隆抗体
目录名称 :
一抗, 单克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
AKT1
价格 :
299美元
price various sizes :
100ug / $299 100ug+Free HRP Secondary BA1054 / $299 100ug+Free Biotin Secondary BA1003 / $299 30ug sample size / $99
克隆性 :
单克隆
克隆编号 :
EBG-1
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
描述 :
Rabbit IgG Monoclonal antibody for P31749 detection. Tested positive for WB IHC IF/IF IP FC in Human Mouse Rat
short description :
Rabbit IgG Monoclonal antibody for P31749 detection. Tested positive for WB IHC IF/IF IP FC in Human Mouse Rat
规格 :
100微克
sample size available :
30ul for $99, contact us for details
UniProt数据库编号 :
P31749
宿主 :
免疫原 :
A synthesized peptide derived from human AKT1 Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
形式 :
Liquid
纯化 :
亲和色谱
储存 :
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4 C short term. Store at -20 C long term. Avoid freeze / thaw cycle.
应用细节 :
免疫印迹1:500~1:2000免疫组化1:50~1:200免疫细胞化学/IF 1:50~1:200免疫沉淀1:50
应用 :
免疫沉淀, IF, 免疫组化, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Immunofluorescent analysis of Hela cells, using AKT1 Antibody. Figure 2. IHC analysis of AKT1 using anti-AKT1 antibody (M00024-1). AKT1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AKT1 Antibody (M00024-1) overnight at 4 C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 1. Western blot analysis of AKT1 using anti-AKT1 antibody (M00024-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 antigen affinity purified polyclonal antibody (Catalog # M00024-1) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for AKT1.
研究种类 :
Apoptosis, Cancer, Cell Cycle, Cell Death, Epigenetics And Nuclear Signaling, Metabolism, Metabolism Processes, Nuclear, Pathways And Processes, Protein Phosphorylation, Ser / Thr Kinases, Serine/Threonine Kinases, Signal Transduction
更新 :
4/22/19 1:33
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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