产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗磷脂酰肌醇(-3)激酶p110甲单克隆抗体
目录 :
M00029
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
BEB-16
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀
图像
图像 1 :
武汉博士德生物工程有限公司 M00029 图像 1
Figure 1. Western blot analysis of PIK3CA using anti-PIK3CA antibody (M00029). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3CA antigen affinity purified polyclonal antibody (Catalog # M00029) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for PIK3CA.
产品信息
SKU号 :
M00029
产品名称 :
抗磷脂酰肌醇(-3)激酶p110甲单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
BEB-16
宿主 :
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
免疫沉淀, IF, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹1:1000~1:2000。 免疫细胞化学/IF 1:50~1:200。 免疫沉淀1:20
描述 :
Boster Bio Anti-PI 3 kinase p110 alpha Monoclonal Antibody catalog # M00029. Tested in IP, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
PIK3CA
UniProt数据库编号 :
P42336
免疫原 :
A synthesized peptide derived from human PI 3 Kinase catalytic subunit alpha
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
背景 :
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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