产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗磷酸化视网膜母细胞瘤(S780)视网膜成神经细胞瘤相关蛋白兔单克隆抗体
目录 :
M00039-1
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
BHC-18
反应物种 :
人类
应用 :
免疫印迹, 免疫组化
图像
图像 1 :
武汉博士德生物工程有限公司 M00039-1 图像 1
Western blot analysis of Rb expression in Jurkat cell lysate treated with Alkaline Phosphatase (M00039-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RB1 monoclonal antibody (Catalog # M00039-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RB1
产品信息
SKU号 :
M00039-1
产品名称 :
抗磷酸化视网膜母细胞瘤(S780)视网膜成神经细胞瘤相关蛋白兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
BHC-18
宿主 :
反应物种 :
人类
应用(s) :
免疫组化, 免疫印迹
应用细节 :
免疫印迹1:500-1:2000。 免疫组化1:50-1:200
描述 :
Boster Bio Anti-Phospho-Retinoblastoma (S780) RB1 Rabbit Monoclonal Antibody catalog # M00039-1. Tested in IHC, WB applications. This antibody reacts with Human.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
视网膜成神经细胞瘤相关蛋白
UniProt数据库编号 :
P06400
免疫原 :
A synthesized peptide derived from human Retinoblastoma
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
Retinoblastoma-associated protein
同义词 :
Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110; RB1;
分子量 :
106159分子量
Protein Function :
Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Subcellular Localization :
Nucleus.
组织特异性 :
Expressed in the retina.
研究种类 :
Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics and Nuclear Signaling, Nucleus, Oncoproteins/Suppressors, Subcellular Markers, Tags & Cell Markers, Transcription, Tumor Suppressors
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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