产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗半胱天冬酶1胱天蛋白酶凋亡相关半胱氨酸肽酶1兔单克隆抗体
目录 :
M00048
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
DCB-3
反应物种 :
人类, 大鼠
应用 :
免疫印迹
图像
图像 1 :
武汉博士德生物工程有限公司 M00048 图像 1
Western blot analysis of Caspase-1 in HeLa cell lysate (M00048). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP1 monoclonal antibody (Catalog # M00048) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP1
图像 2 :
武汉博士德生物工程有限公司 M00048 图像 2
Immunohistochemical analysis of paraffin-embedded human liver, using Caspase-1 Antibody(M00048). CASP1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP1 Antibody (M00048)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
M00048
产品名称 :
抗半胱天冬酶1胱天蛋白酶凋亡相关半胱氨酸肽酶1兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
DCB-3
宿主 :
反应物种 :
人类, 大鼠
应用(s) :
免疫印迹
应用细节 :
免疫印迹1:500-1:2000
描述 :
Boster Bio Anti-Caspase-1 CASP1 Rabbit Monoclonal Antibody catalog # M00048. Tested in WB applications. This antibody reacts with Human, Rat.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
胱天蛋白酶凋亡相关半胱氨酸肽酶1
UniProt数据库编号 :
P29466
免疫原 :
A synthesized peptide derived from human Caspase-1
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
半胱天冬酶1
同义词 :
Caspase-1; CASP-1; 3.4.22.36; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45; Caspase-1 subunit p20; Caspase-1 subunit p10; CASP1; IL1BC, IL1BCE;
分子量 :
45159分子量
Protein Function :
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
Subcellular Localization :
细胞质
组织特异性 :
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
研究种类 :
Apoptosis, Caspases Etc, Cell Biology, Intracellular
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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