产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗ER甲ESR1兔单克隆抗体
目录 :
M00057-2
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
IE-5
反应物种 :
人类, 小鼠, 大鼠, 犬
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 染色质免疫沉淀
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图像
图像 1 :
Western blot analysis of ER alpha expression in (1) MCF7 cell lysate; (2)T47-D cell lysate (M00057-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR1 monoclonal antibody (Catalog # M00057-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR1
图像 2 :
Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using ER alpha Antibody(M00057-2). ESR1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ESR1 Antibody (M00057-2)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
IF analysis of immunocytochemical section of MCF7 cells using anti- ER alpha antibody (M00057-2) . ER alpha was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- ER alpha Antibody (M00057-2) overnight at 4 C. DyLight®488 Conjugated Goat AntiRabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used
产品信息
SKU号 :
M00057-2
产品名称 :
抗ER甲ESR1兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
IE-5
宿主 :
兔
反应物种 :
狗, 人类, 小鼠, 大鼠
应用(s) :
ChIP, 流式细胞仪, IF, 免疫组化, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹1:500-1:2000。 免疫组化1:50-1:100。 免疫细胞化学/IF 1:50-1:100。 ChIP 1:30。 流式细胞仪1:30
描述 :
Boster Bio Anti-ER alpha ESR1 Rabbit Monoclonal Antibody catalog # M00057-2. Tested in ChIP, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Dog, Human, Mouse, Rat.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
ESR1
UniProt数据库编号 :
P03372
免疫原 :
A synthesized peptide derived from human ER alpha
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
雌激素受体
同义词 :
Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1; ESR, NR3A1;
分子量 :
66216分子量
Protein Function :
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA- binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF- kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA- binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.
Subcellular Localization :
Isoform 1: Nucleus. Cytoplasm. Cell membrane ; Peripheral membrane protein ; Cytoplasmic side. A minor fraction is associated with the inner membrane.
组织特异性 :
Widely expressed. Isoform 3 is not expressed in the pituitary gland.
研究种类 :
Cancer, ChIP’ing Antibodies, Endocrine System, Epigenetics and Nuclear Signaling, Neuroscience, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway
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公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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