产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗COX2 PTGS2兔单克隆抗体
目录 :
M00084
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
欧洲经济共同体-16
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Western blot analysis of COX2 expression in RAW2647 cell lysate treated with LPS (M00084). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGS2 monoclonal antibody (Catalog # M00084) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGS2
图像 2 :
IF analysis of immunocytochemical section of Hela cells using anti- COX2 antibody (M00084) . COX2 was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- COX2 Antibody (M00084) overnight at 4 C. DyLight®488 Conjugated Goat AntiRabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
产品信息
SKU号 :
M00084
产品名称 :
抗COX2 PTGS2兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
欧洲经济共同体-16
宿主 :
兔
反应物种 :
人类, 小鼠
应用(s) :
IF, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹1:500-1:1000。 免疫细胞化学/IF 1:50-1:200
描述 :
Boster Bio Anti-COX2 PTGS2 Rabbit Monoclonal Antibody catalog # M00084. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
PTGS2
UniProt数据库编号 :
P35354
免疫原 :
A synthesized peptide derived from human COX2
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
Prostaglandin G/H synthase 2
同义词 :
Prostaglandin G/H synthase 2; 1.14.99.1; Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2; PTGS2; COX2;
分子量 :
68996分子量
Protein Function :
Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.
Subcellular Localization :
Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.
研究种类 :
Metabolic Signaling Pathways, Metabolism, Pathways And Processes, Platelets, Signal Transduction, Signaling Pathway, Tumor Biomarkers
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits