产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗SOX2兔单克隆抗体
目录 :
M00105-1
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
BIO-19
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using SOX2 Antibody(M00105-1). SOX2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SOX2 Antibody (M00105-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 2 :
Western blot analysis of SOX2 expression in (1) NCCIT cell lysate; (2) F9 cell lysate (M00105-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX2 monoclonal antibody (Catalog # M00105-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX2
产品信息
SKU号 :
M00105-1
产品名称 :
抗SOX2兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
BIO-19
宿主 :
兔
反应物种 :
人类, 小鼠
应用(s) :
IF, 免疫组化, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹1:1000-1:2000。 免疫组化1:50-1:200。 免疫细胞化学/IF 1:50-1:200
描述 :
Boster Bio Anti-SOX2 Rabbit Monoclonal Antibody catalog # M00105-1. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
SOX2
UniProt数据库编号 :
P48431
免疫原 :
A synthesized peptide derived from human SOX2
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
转录因子SOX-2
同义词 :
转录因子SOX-2;SOX2;
分子量 :
34310分子量
Protein Function :
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
Subcellular Localization :
Nucleus.
研究种类 :
Cell Type Marker, Developmental Biology, Domain Families, Embryogenesis, Embryonic Stem Cells, Epigenetics and Nuclear Signaling, Germ Cell Markers, Germline Stem Cells, Hlh / Leucine Zipper, Intracellular, Neural Signal Transduction, Neurogenesis, Neurology Process, Neuroscience, Reproduction, Stem Cells, Surface Molecules, Transcription
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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