产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗IKK乙IKBKB兔单克隆抗体
目录 :
M00118-1
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
临别赠品-9
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫沉淀
更多信息或购买 :
图像
图像 1 :
Western blot analysis of IKK beta expression in Daudi cell lysate (M00118-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKBKB monoclonal antibody (Catalog # M00118-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKBKB
产品信息
SKU号 :
M00118-1
产品名称 :
抗IKK乙IKBKB兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
临别赠品-9
宿主 :
兔
反应物种 :
人类, 小鼠
应用(s) :
免疫沉淀, 免疫印迹
应用细节 :
免疫印迹1:500-1:2000。 免疫沉淀1:50
描述 :
Boster Bio Anti-IKK beta IKBKB Rabbit Monoclonal Antibody catalog # M00118-1. Tested in IP, WB applications. This antibody reacts with Human, Mouse.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
IKBKB
UniProt数据库编号 :
O14920
免疫原 :
A synthesized peptide derived from human IKK beta
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
Inhibitor of nuclear factor kappa-B kinase subunit beta
同义词 :
Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; 2.7.11.10; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB; IKBKB; IKKB;
分子量 :
86564分子量
Protein Function :
Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.
Subcellular Localization :
Cytoplasm. Nucleus. Membrane raft. Colocalized with DPP4 in membrane rafts.
组织特异性 :
Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
研究种类 :
Atherosclerosis, Cardiovascular, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Nfkb Pathway, Nuclear Signaling Pathways, Protein Phosphorylation, Ser / Thr Kinases, Signal Transduction, Tlr Signaling, Vascular Inflammation
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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