产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗切断的PARP多聚(ADP-核糖)聚合酶兔单克隆抗体
目录 :
M00122-2
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
BHH-16
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀, 流式细胞仪
图像
图像 1 :
武汉博士德生物工程有限公司 M00122-2 图像 1
Western blot analysis of Cleaved PARP expression in Jurkat cell lysate (M00122-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP1 monoclonal antibody (Catalog # M00122-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP1
产品信息
SKU号 :
M00122-2
产品名称 :
抗切断的PARP多聚(ADP-核糖)聚合酶兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
BHH-16
宿主 :
反应物种 :
人类
应用(s) :
流式细胞仪, 免疫沉淀, IF, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹1:500-1:2000。 免疫细胞化学/IF 1:50-1:200。 免疫沉淀1:50。 流式细胞仪1:50
描述 :
Boster Bio Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody catalog # M00122-2. Tested in Flow Cytometry, IP, IF, ICC, WB applications. This antibody reacts with Human.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
多聚(ADP-核糖)聚合酶
UniProt数据库编号 :
P09874
免疫原 :
A synthesized peptide derived from human Cleaved PARP
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
Poly [ADP-ribose] polymerase 1
同义词 :
Poly [ADP-ribose] polymerase 1; PARP-1; 2.4.2.30; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD (+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1; PARP1; ADPRT, PPOL;
分子量 :
113084分子量
Protein Function :
Involved in the base excision repair (BER) pathway, by catalyzing the poly (ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly (ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
Subcellular Localization :
Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
研究种类 :
Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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