抗SHP2 PTPN11兔单克隆抗体 | 武汉博士德生物工程有限公司 M00150-1 产品信息

产品简要

公司名称 :

武汉博士德生物工程有限公司

产品类型 :

抗体

产品名称 :

抗SHP2 PTPN11兔单克隆抗体

M00150-1

规格 :

100微升

价格 :

315美元

克隆性 :

单克隆

宿主 :

domestic rabbit

共轭标签 :

未共轭

克隆名称 :

GBO-16

反应物种 :

人类, 小鼠, 大鼠

应用 :

免疫印迹, 免疫沉淀

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图像

图像 1 :

Western blot analysis of SHP2 expression in A431 cell lysate (M00150-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN11 monoclonal antibody (Catalog # M00150-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN11

产品信息

SKU号 :

M00150-1

产品名称 :

抗SHP2 PTPN11兔单克隆抗体

价格 :

315美元

规格 :

100微升

克隆性 :

单克隆

克隆编号 :

GBO-16

宿主 :

兔

反应物种 :

人类, 小鼠, 大鼠

应用(s) :

免疫沉淀, 免疫印迹

应用细节 :

免疫印迹1:500-1:1000。免疫沉淀1:20

描述 :

Boster Bio Anti-SHP2 PTPN11 Rabbit Monoclonal Antibody catalog # M00150-1. Tested in IP, WB applications. This antibody reacts with Human, Mouse, Rat.

浓度 :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

基因名称 :

PTPN11

UniProt数据库编号 :

Q06124

免疫原 :

A synthesized peptide derived from human SHP2

形式 :

液体

Contents :

兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA

纯化 :

亲和色谱

储存 :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

基因全称 :

Tyrosine-protein phosphatase non-receptor type 11

同义词 :

Tyrosine-protein phosphatase non-receptor type 11; 3.1.3.48; Protein-tyrosine phosphatase 1D; PTP-1D; Protein-tyrosine phosphatase 2C; PTP-2C; SH-PTP2; SHP-2; Shp2; SH-PTP3; PTPN11; PTP2C, SHPTP2;

分子量 :

68436分子量

Protein Function :

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity.

Subcellular Localization :

细胞质

组织特异性 :

Widely expressed, with highest levels in heart, brain, and skeletal muscle.

研究种类 :

Neuroscience, Protein Phosphorylation, Sensory System, Signal Transduction, Tyrosine Phosphatases

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