产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗小窝蛋白1 CAV1兔单克隆抗体
目录 :
M00179
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
GE-3
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
图像
图像 1 :
武汉博士德生物工程有限公司 M00179 图像 1
Immunohistochemical analysis of paraffin-embedded mouse lung, using Caveolin-1 Antibody(M00179). CAV1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CAV1 Antibody (M00179)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 2 :
武汉博士德生物工程有限公司 M00179 图像 2
Western blot analysis of Caveolin-1 expression in A431 cell lysate (M00179). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAV1 monoclonal antibody (Catalog # M00179) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAV1
产品信息
SKU号 :
M00179
产品名称 :
抗小窝蛋白1 CAV1兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
GE-3
宿主 :
反应物种 :
人类
应用(s) :
IF, 免疫组化, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹1:500-1:2000。 免疫组化1:50-1:200。 免疫细胞化学/IF 1:50-1:200
描述 :
Boster Bio Anti-Caveolin-1 CAV1 Rabbit Monoclonal Antibody catalog # M00179. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
CAV1
UniProt数据库编号 :
Q03135
免疫原 :
A synthesized peptide derived from human Caveolin-1
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
小窝蛋白1
同义词 :
小窝蛋白1;CAV1;CAV;
分子量 :
20472分子量
Protein Function :
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)- mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3- dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
Subcellular Localization :
Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin- like structure in the membrane. Membrane protein of caveolae.
组织特异性 :
Expressed in muscle and lung, less so in liver, brain and kidney.
研究种类 :
Calcium Channels, Calcium Signaling, Cancer, Cancer Metabolism, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Neuroscience, Neurotransmission, Pathways And Processes, Signal Transduction, Signaling Pathway
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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