产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗Ki67抗原MKI67抗体
目录 :
M00254-4
规格 :
100微升
价格 :
299美元
克隆性 :
多克隆
宿主 :
鸡
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Western blot analysis of equal amounts of cell lysates probed with chicken pAb to Ki-67, M00254-4, dilution 1:5,000, in green: [1] protein standard (red), [2] HeLa cells, [3] HEK293 cells. Strong double bands above 250kDa correspond to the two major isoforms of molecular weight 345kDa and 395kDa of Ki-67. Since Ki-67 is a rather unstable multiple protein proteolytic fragments of these isoforms are also detected on the blot.
图像 2 :
Immunofluorescent analysis of HeLa cells stained with chicken pAb to Ki-67, M00254-4, dilution 1:2,000 in red, and mouse mAb to ?-tubulin
产品信息
SKU号 :
M00254-4
产品名称 :
抗Ki67抗原MKI67抗体
价格 :
299美元
规格 :
100微升
克隆性 :
多克隆
宿主 :
鸡
反应物种 :
人类, 大鼠, 小鼠
应用(s) :
免疫印迹, IF, 免疫细胞化学
应用细节 :
WB: 1:5, 000。 IF/ICC: 1:1, 000-5, 000
描述 :
Boster Bio Anti-Ki-67 MKI67 Antibody catalog # M00254-4. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
MKI67
UniProt数据库编号 :
P46013
免疫原 :
Mixture of recombinant human Ki-67 constructs expressing the N-terminal region, amino acids 1-300 and an internal region, amino acids 1111-1490
Contents :
Supplied as an aliquot of IgY preparation plus 5mM NaN 3
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
Proliferation marker protein Ki-67
同义词 :
Proliferation marker protein Ki-67 ; Antigen identified by monoclonal antibody Ki-67 ; Antigen KI-67 ; Antigen Ki67 ; MKI67 ;
分子量 :
358694分子量
Protein Function :
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Subcellular Localization :
Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
研究种类 :
Cancer, Cell Biology, Cell Cycle, Cell Division, Cell Type Marker, Cell Type Markers, Neurogenesis, Neurology Process, Neuron Marker, Neuroscience, Tags & Cell Markers, Tumor Biomarkers
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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