抗Chk2 CHEK2兔单克隆抗体 | 武汉博士德生物工程有限公司 M00277 产品信息

产品简要

公司名称 :

武汉博士德生物工程有限公司

产品类型 :

抗体

产品名称 :

抗Chk2 CHEK2兔单克隆抗体

M00277

规格 :

100微升

价格 :

315美元

克隆性 :

单克隆

宿主 :

domestic rabbit

共轭标签 :

未共轭

克隆名称 :

FOF-3

反应物种 :

人类

应用 :

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

更多信息或购买 :

武汉博士德生物工程有限公司产品网页

图像

图像 1 :

Western blot analysis of Chk2 expression in (1) HeLa cell lysate; (2) 293T cell lysate (M00277). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK2 monoclonal antibody (Catalog # M00277) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHEK2

产品信息

SKU号 :

M00277

产品名称 :

抗Chk2 CHEK2兔单克隆抗体

价格 :

315美元

规格 :

100微升

克隆性 :

单克隆

克隆编号 :

FOF-3

宿主 :

兔

反应物种 :

人类

应用(s) :

免疫沉淀, IF, 免疫组化, 免疫细胞化学, 免疫印迹

应用细节 :

免疫印迹1:10000-1:20000。免疫组化1:50-1:200。免疫细胞化学/IF 1:50-1:200。免疫沉淀1:50

描述 :

Boster Bio Anti-Chk2 CHEK2 Rabbit Monoclonal Antibody catalog # M00277. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human.

浓度 :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

基因名称 :

CHEK2

UniProt数据库编号 :

O96017

免疫原 :

A synthesized peptide derived from human Chk2

形式 :

液体

Contents :

兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA

纯化 :

亲和色谱

储存 :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

基因全称 :

丝氨酸/苏氨酸蛋白激酶Chk2

同义词 :

Serine/threonine-protein kinase Chk2; 2.7.11.1; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2; CHEK2; CDS1, CHK2, RAD53;

分子量 :

60915分子量

Protein Function :

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X- R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (PubMed:25361978).

Subcellular Localization :

Isoform 2: Nucleus. Isoform 10 is present throughout the cell.

组织特异性 :

High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

研究种类 :

Cancer, Cell Cycle, DNA / RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling, Kinases/Phosphatases

更多信息或购买 :

武汉博士德生物工程有限公司产品网页

公司信息