产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗53BP1兔单克隆抗体
目录 :
M00397
规格 :
100 ug/vial
价格 :
299美元
克隆性 :
单克隆
宿主 :
共轭标签 :
nonconjugated
克隆名称 :
ABAI-20
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学
图像
图像 1 :
武汉博士德生物工程有限公司 M00397 图像 1
Western blot analysis of 53BP1 expression in HeLa cell lysate (M00397). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53BP1 monoclonal antibody (Catalog # M00397) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53BP1
产品信息
SKU号 :
M00397
状态 :
Enabled
名称 :
抗53BP1兔单克隆抗体
目录名称 :
一抗, 单克隆抗体, 兔单克隆抗体, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
TP53BP1
价格 :
299美元
price various sizes :
100ug / $299 100ug+Free HRP Secondary BA1054 / $299 100ug+Free Biotin Secondary BA1003 / $299 30ug sample size / $99
克隆性 :
单克隆
克隆编号 :
ABAI-20
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油
描述 :
Monoclonal antibody for 53BP1/TP53BP1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat 53BP1/TP53BP1 information: Molecular Weight: 213574 MW; Subcellular Localization: Nucleus. Chromosome, centromere, kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. H4K20me2 is required for efficient localization to double strand breaks and removal of proteins that have a high affinity for H4K20me2 such as L3MBTL1 and KDM4A is needed.
short description :
Anti-53BP1 Rabbit Monoclonal Antibody tested for Flow Cytometry, IF, IHC, ICC, WB in Human, Mouse, Rat
规格 :
100 ug/vial
sample size available :
30ul for $99, contact us for details
UniProt数据库编号 :
Q12888
宿主 :
免疫原 :
A synthesized peptide derived from human 53BP1
形式 :
Liquid
纯化 :
亲和色谱
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
应用细节 :
免疫印迹1:500-1:2000。 免疫组化1:50-1:200。 免疫细胞化学/IF 1:50-1:200。 流式细胞仪1:50
应用 :
IF, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Western blot analysis of 53BP1 expression in HeLa cell lysate (M00397). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53BP1 monoclonal antibody (Catalog # M00397) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53BP1
研究种类 :
Cancer, Dna / Rna, Dna Damage & Repair, Dna Damage Response, Epigenetics And Nuclear Signaling, Oncoproteins/Suppressors, P53 Pathway, Tumor Suppressors
同义词 :
Tumor suppressor p53-binding protein 1;53BP1;p53-binding protein 1;p53BP1;TP53BP1;
基因全称 :
Tumor suppressor p53-binding protein 1
分子量 :
213574分子量
protein function :
Plays a key role in the response to DNA damage. May have a role in checkpoint signaling during mitosis. Enhances TP53- mediated transcriptional activation.
subcellular localization :
Nucleus. Chromosome, centromere, kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. H4K20me2 is required for efficient localization to double strand breaks and removal of proteins that have a high affinity for H4K20me2 such as L3MBTL1 and KDM4A is needed.
更新 :
4/22/19 1:33
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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