抗SNF5 SMARCB1兔单克隆抗体 | 武汉博士德生物工程有限公司 M00500 产品信息

产品简要

公司名称 :

武汉博士德生物工程有限公司

产品类型 :

抗体

产品名称 :

抗SNF5 SMARCB1兔单克隆抗体

M00500

规格 :

100微升

价格 :

315美元

克隆性 :

单克隆

宿主 :

domestic rabbit

共轭标签 :

未共轭

克隆名称 :

AFO-19

反应物种 :

人类, 小鼠, 大鼠

应用 :

免疫印迹, 免疫沉淀

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图像

图像 1 :

Western blot analysis of SNF5 in (1) HeLa cell lysate; (2) K562 cell lysate (M00500). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCB1 monoclonal antibody (Catalog # M00500) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCB1

产品信息

SKU号 :

M00500

产品名称 :

抗SNF5 SMARCB1兔单克隆抗体

价格 :

315美元

规格 :

100微升

克隆性 :

单克隆

克隆编号 :

AFO-19

宿主 :

兔

反应物种 :

人类, 小鼠, 大鼠

应用(s) :

免疫沉淀, 免疫印迹

应用细节 :

免疫印迹1:500-1:2000。免疫沉淀1:50

描述 :

Boster Bio Anti-SNF5 SMARCB1 Rabbit Monoclonal Antibody catalog # M00500. Tested in IP, WB applications. This antibody reacts with Human, Mouse, Rat.

浓度 :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

基因名称 :

SMARCB1

UniProt数据库编号 :

Q12824

免疫原 :

A synthesized peptide derived from human SNF5

形式 :

液体

Contents :

兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA

纯化 :

亲和色谱

储存 :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

基因全称 :

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1

同义词 :

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; BRG1-associated factor 47; BAF47; Integrase interactor 1 protein; SNF5 homolog; hSNF5; SMARCB1; BAF47, INI1, SNF5L1;

分子量 :

44141分子量

Protein Function :

Core component of the BAF (hSWI/SNF) complex. This ATP- dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors- specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1.

Subcellular Localization :

Nucleus.

研究种类 :

Tumor Suppressors

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