产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-ErbB (HER3) Rabbit Monoclonal Antibody
目录 :
M00539
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
BIB-5
反应物种 :
人类, 大鼠
应用 :
免疫印迹, 免疫沉淀
图像
图像 1 :
武汉博士德生物工程有限公司 M00539 图像 1
Western blot analysis of ErbB (HER3) expression in MCF-7 cell lysate (M00539). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB3 monoclonal antibody (Catalog # M00539) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERBB3
产品信息
SKU号 :
M00539
产品名称 :
Anti-ErbB (HER3) Rabbit Monoclonal Antibody
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
BIB-5
宿主 :
反应物种 :
人类, 大鼠
应用(s) :
免疫印迹, 免疫沉淀
应用细节 :
免疫印迹1:500-1:2000。 免疫沉淀1:30
描述 :
Boster Bio Anti-ErbB (HER3) Rabbit Monoclonal Antibody catalog # M00539. Tested in IP, WB applications. This antibody reacts with Human, Rat.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
ERBB3
UniProt数据库编号 :
P21860
免疫原 :
A synthesized peptide derived from human ErbB (HER3)
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
Receptor tyrosine-protein kinase erbB-3
同义词 :
Receptor tyrosine-protein kinase erbB-3; 2.7.10.1; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3; ERBB3; HER3;
分子量 :
148098分子量
Protein Function :
Binds and is activated by neuregulins and NTAK. May also be activated by CSPG5.
Subcellular Localization :
Isoform 1: Cell membrane; Single-pass type I membrane protein.
组织特异性 :
Epithelial tissues and brain.
研究种类 :
Apoptosis, Cell Biology, Developmental Biology, Growth Factors/Hormones, Intracellular, Kinases, Nervous System Development, Organogenesis, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tyrosine Kinases
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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