免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
图像 1 :
Western blot analysis of Calreticulin expression in T47 D cell lysate (M00541-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGR monoclonal antibody (Catalog # M00541-1) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGR
免疫沉淀, IF, 免疫组化, 免疫细胞化学, 免疫印迹
免疫印迹1:1000-1:2000。 免疫组化1:50-1:200。 免疫细胞化学/IF 1:50-1:200。 免疫沉淀1:50
Boster Bio Anti-Progesterone Receptor PGR Rabbit Monoclonal Antibody catalog # M00541-1. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human.
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
A synthesized peptide derived from human Progesterone Receptor
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Progesterone receptor; PR; Nuclear receptor subfamily 3 group C member 3; PGR; NR3C3;
Protein Function :
The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Subcellular Localization :
Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G (1) and G (2)/M phases.
Cancer, Domain Families, Endocrine System, Epigenetics and Nuclear Signaling, Neuroscience, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, Transcription, Tumor Biomarkers, Zinc Finger
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