产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗GATA3兔单克隆抗体
目录 :
M00593
规格 :
100 ug/vial
价格 :
299美元
克隆性 :
单克隆
宿主 :
共轭标签 :
nonconjugated
克隆名称 :
ABBH-7
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 染色质免疫沉淀
图像
图像 1 :
武汉博士德生物工程有限公司 M00593 图像 1
Western blot analysis of GATA3 expression in (1) SH-SY5Y cell lysate; (2) RAW 2647 cell lysate (M00593). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 monoclonal antibody (Catalog # M00593) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA3
图像 2 :
武汉博士德生物工程有限公司 M00593 图像 2
IHC analysis of GATA3 using anti-GATA3 antibody (M00593) on human bladder cancer. GATA3 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GATA3 Antibody (M00593) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
M00593
状态 :
Enabled
名称 :
抗GATA3兔单克隆抗体
目录名称 :
一抗, 单克隆抗体, 兔单克隆抗体, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
GATA3
价格 :
299美元
price various sizes :
100ug / $299 100ug+Free HRP Secondary BA1054 / $299 100ug+Free Biotin Secondary BA1003 / $299 30ug sample size / $99
克隆性 :
单克隆
克隆编号 :
ABBH-7
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油
描述 :
Monoclonal antibody for GATA-3/GATA3 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: ChIP, Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat GATA-3/GATA3 information: Molecular Weight: 47916 MW; Subcellular Localization: Nucleus; Tissue Specificity: T-cells and endothelial cells.
short description :
Anti-GATA3 Rabbit Monoclonal Antibody tested for ChIP, Flow Cytometry, IF, IHC, ICC, WB in Human
规格 :
100 ug/vial
sample size available :
30ul for $99, contact us for details
UniProt数据库编号 :
P23771
宿主 :
免疫原 :
A synthesized peptide derived from human GATA3
形式 :
Liquid
纯化 :
亲和色谱
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
应用细节 :
免疫印迹1:500-1:2000。 免疫组化1:50-1:200。 免疫细胞化学/IF 1:50-1:200。 流式细胞仪1:100。 ChIP
应用 :
ChIP, 流式细胞仪, IF, 免疫组化, 免疫细胞化学, 免疫印迹
反应物种 :
人类
图像标记 :
Western blot analysis of GATA3 expression in (1) SH-SY5Y cell lysate; (2) RAW 2647 cell lysate (M00593). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 monoclonal antibody (Catalog # M00593) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA3 IHC analysis of GATA3 using anti-GATA3 antibody (M00593) on human bladder cancer. GATA3 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GATA3 Antibody (M00593) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
研究种类 :
Adaptive Immunity, Chip'Ing Antibodies, Developmental Biology, Domain Families, Epigenetics And Nuclear Signaling, Immunology, Lineage Markers, Lineage Specification, Non-Cd, Stem Cells, T Cells, Transcription, Zinc Finger
同义词 :
Trans-acting T-cell-specific transcription factor GATA-3;GATA-binding factor 3;GATA3;
基因全称 :
Trans-acting T-cell-specific transcription factor GATA-3
分子量 :
47916分子量
protein function :
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.
subcellular localization :
Nucleus.
组织特异性 :
T细胞和内皮细胞
更新 :
4/30/19 1:13
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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