IF analysis of CD3E and CD68 using anti-CD3E antibody (PB9093) and anti-CD68 antibody (M00602) . CD3E and CD68 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti- CD3E Antibody (PB9093) and mouse anti CD68 Antibody(M00602) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) and Biotin conjugated goat anti-rabbit IgG (BA1003) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The tissue section was developed using Cy3 Conjugated Avidin (BA1037). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of CD68 and CD45 using anti-CD68 antibody (M00602) and anti-CD45 antibody (PB9785) . CD68 and CD45 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL mouse anti- CD68 Antibody (M00602) and rabbit anti CD45 Antibody(PB9785) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) and Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The tissue section was developed using Cy3 Conjugated Avidin (BA1037). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.