产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗SIRT6兔单克隆抗体
目录 :
M00611
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
HBA-19
反应物种 :
人类
应用 :
免疫印迹
更多信息或购买 :
图像
图像 1 :
Western blot analysis of SIRT6 expression in Jurkat cell lysate (M00611). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT6 monoclonal antibody (Catalog # M00611) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT6
产品信息
SKU号 :
M00611
产品名称 :
抗SIRT6兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
HBA-19
宿主 :
兔
反应物种 :
人类
应用(s) :
免疫印迹
应用细节 :
免疫印迹1:1000-1:2000
描述 :
Boster Bio Anti-SIRT6 Rabbit Monoclonal Antibody catalog # M00611. Tested in WB applications. This antibody reacts with Human.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
SIRT6
UniProt数据库编号 :
Q8N6T7
免疫原 :
A synthesized peptide derived from human SIRT6
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
NAD依赖的蛋白脱乙酰基酶沉默调节蛋白6
同义词 :
NAD-dependent protein deacetylase sirtuin-6; 3.5.1.-; Regulatory protein SIR2 homolog 6; SIR2-like protein 6; SIRT6; SIR2L6;
分子量 :
39119分子量
Protein Function :
NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD (+) in the absence of acetylated substrate.
Subcellular Localization :
Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
研究种类 :
Acetylation, Apoptosis, Atherosclerosis, Cancer, Cardiovascular, ChIP’ing Antibodies, Chromatin Modifying Enzymes, Diabetes Associated, Epigenetics and Nuclear Signaling, Metabolism, Nfkb Pathway, Nuclear Signaling Pathways, Obesity, Signal Transduction
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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