产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗MUC5AC/粘蛋白5Ac兔单克隆抗体
目录 :
M00612-1
规格 :
100 ug/vial
价格 :
299美元
克隆性 :
单克隆
宿主 :
共轭标签 :
nonconjugated
克隆名称 :
ABAD-13
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
图像
图像 1 :
武汉博士德生物工程有限公司 M00612-1 图像 1
Western blot analysis of MUC5AC expression in human stomach lysate (M00612-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC5AC monoclonal antibody (Catalog # M00612-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC5AC
产品信息
SKU号 :
M00612-1
状态 :
Enabled
名称 :
抗MUC5AC/粘蛋白5Ac兔单克隆抗体
目录名称 :
一抗, 单克隆抗体, 兔单克隆抗体, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
MUC5AC
价格 :
299美元
price various sizes :
100ug / $299 100ug+Free HRP Secondary BA1054 / $299 100ug+Free Biotin Secondary BA1003 / $299 30ug sample size / $99
克隆性 :
单克隆
克隆编号 :
ABAD-13
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油
描述 :
Monoclonal antibody for MUCIN 5AC/MUC5AC detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human MUCIN 5AC/MUC5AC information: Molecular Weight: 585570 MW; Subcellular Localization: Secreted ; Tissue Specificity: Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum.
short description :
Anti-MUC5AC Rabbit Monoclonal Antibody tested for IF, IHC, ICC, WB in Human
规格 :
100 ug/vial
sample size available :
30ul for $99, contact us for details
UniProt数据库编号 :
P98088
宿主 :
免疫原 :
A synthesized peptide derived from human MUC5AC
形式 :
Liquid
纯化 :
亲和色谱
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
应用细节 :
免疫印迹1:500-1:2000。 免疫组化1:50-1:200。 免疫细胞化学/IF 1:50-1:200
应用 :
IF, 免疫组化, 免疫细胞化学, 免疫印迹
反应物种 :
人类
图像标记 :
Western blot analysis of MUC5AC expression in human stomach lysate (M00612-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC5AC monoclonal antibody (Catalog # M00612-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC5AC
研究种类 :
Cancer, Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Tumor Antigens, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens
同义词 :
Mucin-5AC ;MUC-5AC ;Gastric mucin ;Lewis B blood group antigen ;LeB ;Major airway glycoprotein ;Mucin-5 subtype AC, tracheobronchial;Tracheobronchial mucin ;TBM ;MUC5AC ;MUC5 ;
基因全称 :
粘蛋白5AC
分子量 :
585570分子量
protein function :
Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.
subcellular localization :
Secreted .
组织特异性 :
Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum.
更新 :
4/22/19 1:33
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits