产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗RANTES单克隆抗体
目录 :
M00617
规格 :
100微克
价格 :
299美元
克隆性 :
单克隆
宿主 :
共轭标签 :
nonconjugated
克隆名称 :
ADHH-3
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学
图像
图像 1 :
武汉博士德生物工程有限公司 M00617 图像 1
Figure 1. Western blot analysis of CCL5 using anti-CCL5 antibody (M00617) in RANTES recombinant protein lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL5 antigen affinity purified polyclonal antibody (Catalog # M00617) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CCL5.
产品信息
SKU号 :
M00617
状态 :
Enabled
名称 :
抗RANTES单克隆抗体
目录名称 :
单克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
CCL5
价格 :
299美元
price various sizes :
100ug / $299 100ug+Free HRP Secondary BA1054 / $299 100ug+Free Biotin Secondary BA1003 / $299 30ug sample size / $99
克隆性 :
单克隆
克隆编号 :
ADHH-3
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油
描述 :
Rabbit IgG Monoclonal antibody for RANTES detection. Tested positive for WB, ICC/IF in Human
short description :
Rabbit IgG Monoclonal antibody for RANTES detection. Tested positive for WB, ICC/IF in Human
规格 :
100微克
sample size available :
30ul for $99, contact us for details
UniProt数据库编号 :
P13501
宿主 :
免疫原 :
A synthesized peptide derived from human RANTES.
形式 :
Liquid
纯化 :
亲和色谱
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
应用细节 :
免疫印迹1:1000~1:5000。 免疫细胞化学/IF 1:50~1:200
应用 :
IF, 免疫印迹
反应物种 :
人类
图像标记 :
Figure 1. Western blot analysis of CCL5 using anti-CCL5 antibody (M00617) in RANTES recombinant protein lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL5 antigen affinity purified polyclonal antibody (Catalog # M00617) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CCL5.
研究种类 :
Antiviral Signaling, Atherosclerosis, Cardiovascular, Chemokines, Immune System Diseases, Immunology, Innate Immunity, Vascular Inflammation
更新 :
4/25/19 20:34
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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