产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Sumo 1 Rabbit Monoclonal Antibody
目录 :
M00631-1
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
BFI-19
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
图像
图像 1 :
武汉博士德生物工程有限公司 M00631-1 图像 1
Western blot analysis of SUMO1 expression in HeLa cell lysate (M00631-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUMO1 monoclonal antibody (Catalog # M00631-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUMO1
图像 2 :
武汉博士德生物工程有限公司 M00631-1 图像 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Sumo 1 Antibody (M00631-1). SUMO1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SUMO1 Antibody (M00631-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
M00631-1
产品名称 :
Anti-Sumo 1 Rabbit Monoclonal Antibody
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
BFI-19
宿主 :
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
流式细胞仪, 免疫沉淀, IF, 免疫组化, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹1:500-1:2000。 免疫组化1:50-1:200。 免疫细胞化学/IF 1:50-1:200。 免疫沉淀1:50。 流式细胞仪1:50
描述 :
Boster Bio Anti-Sumo 1 Rabbit Monoclonal Antibody catalog # M00631-1. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
泛素相关的修饰因子-1
UniProt数据库编号 :
P63165
免疫原 :
A synthesized peptide derived from human Sumo 1
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
Small ubiquitin-related modifier 1
同义词 :
Small ubiquitin-related modifier 1; SUMO-1; GAP-modifying protein 1; GMP1; SMT3 homolog 3; Sentrin; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein SMT3C; Smt3C; Ubiquitin-like protein UBL1; SUMO1; SMT3C, SMT3H3, UBL1; OK/SW-cl.43;
分子量 :
11557分子量
Protein Function :
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1 (PubMed:19223394). Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.
Subcellular Localization :
Nucleus membrane. Nucleus speckle. Cytoplasm. Nucleus, PML body. Cell membrane. Recruited by BCL11A into the nuclear body.
研究种类 :
Cancer, Cardiovascular, Cell Biology, Cell Death, Metabolism, Metabolism Processes, Pathways And Processes, Proteasome / Ubiquitin, Proteolysis / Ubiquitin
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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