抗DKK1兔单克隆抗体 | 武汉博士德生物工程有限公司 M00632 产品信息

产品简要

公司名称 :

武汉博士德生物工程有限公司

产品类型 :

抗体

产品名称 :

抗DKK1兔单克隆抗体

M00632

规格 :

100微升

价格 :

315美元

克隆性 :

单克隆

宿主 :

domestic rabbit

共轭标签 :

未共轭

克隆名称 :

FHG-4

反应物种 :

人类, 大鼠

应用 :

免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

更多信息或购买 :

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图像

图像 1 :

Western blot analysis of DKK1 expression in HeLa cell lysate (M00632). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK1 monoclonal antibody (Catalog # M00632) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DKK1

产品信息

SKU号 :

M00632

产品名称 :

抗DKK1兔单克隆抗体

价格 :

315美元

规格 :

100微升

克隆性 :

单克隆

克隆编号 :

FHG-4

宿主 :

兔

反应物种 :

人类, 大鼠

应用(s) :

流式细胞仪, IF, 免疫组化, 免疫细胞化学, 免疫印迹

应用细节 :

免疫印迹1:1000-1:2000。免疫组化1:50-1:200。免疫细胞化学/IF 1:50-1:200。流式细胞仪1:50

描述 :

Boster Bio Anti-DKK1 Rabbit Monoclonal Antibody catalog # M00632. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat.

浓度 :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

基因名称 :

DKK1

UniProt数据库编号 :

O94907

免疫原 :

A synthesized peptide derived from human DKK1

形式 :

液体

Contents :

兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA

纯化 :

亲和色谱

储存 :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

基因全称 :

Dickkopf-related protein 1

同义词 :

Dickkopf-related protein 1; Dickkopf-1; Dkk-1; hDkk-1; SK; DKK1; UNQ492/PRO1008;

分子量 :

28672分子量

Protein Function :

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

Subcellular Localization :

Secreted.

组织特异性 :

胎盘

研究种类 :

Cardiovascular, Developmental Biology, Embryogenesis, Immunology, Secreted, Signaling Pathways, Stem Cells

更多信息或购买 :

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公司信息