产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗钠钾ATPase ATP1A1兔单克隆抗体
目录 :
M00956
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
AID-1
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
图像
图像 1 :
武汉博士德生物工程有限公司 M00956 图像 1
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma, using Sodium Potassium ATPase Antibody(M00956). ATP1A1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ATP1A1 Antibody (M00956)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 2 :
武汉博士德生物工程有限公司 M00956 图像 2
Western blot analysis of Sodium Potassium ATPase expression in HeLa cell lysate (M00956). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1A1 monoclonal antibody (Catalog # M00956) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1A1
产品信息
SKU号 :
M00956
产品名称 :
抗钠钾ATPase ATP1A1兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
AID-1
宿主 :
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
流式细胞仪, IF, 免疫组化, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹1:5000~1:10000。 免疫组化1:50~1:100。 免疫细胞化学/IF 1:50~1:200。 FC 1:50
描述 :
Boster Bio Anti-Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody catalog # M00956. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
ATP1A1
UniProt数据库编号 :
P05023
免疫原 :
A synthesized peptide derived from human Sodium Potassium ATPase
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
Sodium/potassium-transporting ATPase subunit alpha-1
同义词 :
Sodium/potassium-transporting ATPase subunit alpha-1; Na (+)/K (+) ATPase alpha-1 subunit; 3.6.3.9; Sodium pump subunit alpha-1; ATP1A1;
分子量 :
112896分子量
Protein Function :
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Subcellular Localization :
Cell membrane; Multi-pass membrane protein. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
研究种类 :
Atpases, Cancer, Cancer Metabolism, Cell Type Marker, Metabolism, Neurogenesis, Neurology Process, Neuron Marker, Neuroscience, Plasma Membrane, Response To Hypoxia, Signal Transduction, Subcellular Markers, Synapse Marker, Tags & Cell Markers
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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