产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗IRF5兔单克隆抗体
目录 :
M00958
规格 :
100 ug/vial
价格 :
299美元
克隆性 :
单克隆
宿主 :
共轭标签 :
nonconjugated
克隆名称 :
GHI-9
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀
图像
图像 1 :
武汉博士德生物工程有限公司 M00958 图像 1
Western blot analysis of IRF5 expression in THP-1 cell lysate (M00958). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF5 monoclonal antibody (Catalog # M00958) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF5
产品信息
SKU号 :
M00958
状态 :
Enabled
名称 :
抗IRF5兔单克隆抗体
目录名称 :
一抗, 单克隆抗体, 兔单克隆抗体, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
IRF5
价格 :
299美元
price various sizes :
100ug / $299 100ug+Free HRP Secondary BA1054 / $299 100ug+Free Biotin Secondary BA1003 / $299 30ug sample size / $99
克隆性 :
单克隆
克隆编号 :
GHI-9
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油
描述 :
Monoclonal antibody for IRF 5/IRF5 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat IRF 5/IRF5 information: Molecular Weight: 56044 MW; Subcellular Localization: Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
short description :
Anti-IRF5 Rabbit Monoclonal Antibody tested for Flow Cytometry, IP, IF, IHC, ICC, WB in Human, Mouse, Rat
规格 :
100 ug/vial
sample size available :
30ul for $99, contact us for details
UniProt数据库编号 :
Q13568
宿主 :
免疫原 :
A synthesized peptide derived from human IRF5
形式 :
Liquid
纯化 :
亲和色谱
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
应用细节 :
免疫印迹1:500-1:1000。 免疫组化1:50-1:200。 免疫细胞化学/IF 1:100-1:500。 免疫沉淀1:100。 流式细胞仪1:50
应用 :
免疫沉淀, IF, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Western blot analysis of IRF5 expression in THP-1 cell lysate (M00958). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF5 monoclonal antibody (Catalog # M00958) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF5
研究种类 :
Cytokines, Domain Families, Epigenetics And Nuclear Signaling, Hlh / Leucine Zipper, Immunology, Innate Immunity, Interferons, Polymerase Associated Factors, Transcription, Transcription Factors
同义词 :
干扰素调节因子5;IRF-5;IRF5;
基因全称 :
干扰素调节因子5
分子量 :
56044分子量
protein function :
Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling.
subcellular localization :
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
更新 :
4/22/19 1:33
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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