产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗TSG101兔单克隆抗体
目录 :
M01233
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
IDG-20
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
图像
图像 1 :
武汉博士德生物工程有限公司 M01233 图像 1
Western blot analysis of TSG101 expression in Jurkat cell lysate (M01233). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG101 monoclonal antibody (Catalog # M01233) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSG101
图像 2 :
武汉博士德生物工程有限公司 M01233 图像 2
Western blot analysis of TSG101 expression in NIH/3T3 cell lysate (Lane 1),: Hela cell lysate (Lane 2), K562 cell lysate (Lane 3), Jurkat cell lysate (Lane 4), A431 cell lysate (Lane 5)(M01233). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG101 monoclonal antibody (Catalog # M01233) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSG101.
图像 3 :
武汉博士德生物工程有限公司 M01233 图像 3
IF analysis of immunocytochemical section of A431 cell using anti- TSG101 antibody (M01233) . TSG101 was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- TSG101 Antibody (M01233) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
产品信息
SKU号 :
M01233
产品名称 :
抗TSG101兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
IDG-20
宿主 :
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
流式细胞仪, IF, 免疫组化, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹1:500-1:2000。 免疫组化1:50-1:200。 免疫细胞化学/IF 1:50-1:200。 流式细胞仪1:50
描述 :
Boster Bio Anti-TSG101 Rabbit Monoclonal Antibody catalog # M01233. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
TSG101
UniProt数据库编号 :
Q99816
免疫原 :
A synthesized peptide derived from human TSG101
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
Tumor susceptibility gene 101 protein
同义词 :
Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101; TSG101;
分子量 :
43944分子量
Protein Function :
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
Subcellular Localization :
Cytoplasm. Membrane; Peripheral membrane protein. Nucleus. Late endosome membrane; Peripheral membrane protein. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
组织特异性 :
Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
研究种类 :
Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Cell Differentiation, Epigenetics and Nuclear Signaling, Host Virus Interaction, Interspecies Interaction, Microbiology
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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