免疫印迹, 免疫组化, 流式细胞仪
图像 1 :
Immunohistochemical analysis of paraffin-embedded human colon using Pax2 Antibody(M01288-1). PAX2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PAX2 Antibody (M01288-1)overnight at 4â„ƒ. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37â„ƒ. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 2 :
Western blot analysis of Pax2 expression in Human fetal kidney lysate (M01288-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX2 monoclonal antibody (Catalog # M01288-1) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX2
流式细胞仪, 免疫组化, 免疫印迹
免疫印迹1:500-1:2000。 免疫组化1:50-1:200。 流式细胞仪1:50
Boster Bio Anti-Pax2 Rabbit Monoclonal Antibody catalog # M01288-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse.
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
A synthesized peptide derived from human Pax2
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Paired box protein Pax-2
Paired box protein Pax-2; PAX2;
Protein Function :
Transcription factor that may have a role in kidney cell differentiation (PubMed:24676634). Has a critical role in the development of the urogenital tract, the eyes, and the CNS.
Subcellular Localization :
Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.
Cell Type Marker, Developmental Biology, Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Neuroscience, Organogenesis, Transcription, Transcription Factors
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
Premium Provider of Antibodies and ELISA Kits