抗血栓调节蛋白THBD兔单克隆抗体 | 武汉博士德生物工程有限公司 M01325-1 产品信息

产品简要

公司名称 :

武汉博士德生物工程有限公司

产品类型 :

抗体

产品名称 :

抗血栓调节蛋白THBD兔单克隆抗体

M01325-1

规格 :

100微升

价格 :

315美元

克隆性 :

单克隆

宿主 :

domestic rabbit

共轭标签 :

未共轭

克隆名称 :

COO-20

反应物种 :

人类

应用 :

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

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图像

图像 1 :

Immunohistochemical analysis of paraffin-embedded human bladder, using Thrombomodulin Antibody(M01325-1). THBD was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-THBD Antibody (M01325-1)overnight at 4â„ƒ. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37â„ƒ. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

图像 2 :

Western blot analysis of Thrombomodulin expression in human placenta lysate (M01325-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THBD monoclonal antibody (Catalog # M01325-1) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THBD

产品信息

SKU号 :

M01325-1

产品名称 :

抗血栓调节蛋白THBD兔单克隆抗体

价格 :

315美元

规格 :

100微升

克隆性 :

单克隆

克隆编号 :

COO-20

宿主 :

兔

反应物种 :

人类

应用(s) :

免疫沉淀, IF, 免疫组化, 免疫细胞化学, 免疫印迹

应用细节 :

免疫印迹1:500-1:2000。免疫组化1:50-1:200。免疫细胞化学/IF 1:50-1:200。免疫沉淀1:50

描述 :

Boster Bio Anti-Thrombomodulin THBD Rabbit Monoclonal Antibody catalog # M01325-1. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human.

浓度 :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

基因名称 :

THBD

UniProt数据库编号 :

P07204

免疫原 :

A synthesized peptide derived from human Thrombomodulin

形式 :

液体

Contents :

兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA

纯化 :

亲和色谱

储存 :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

基因全称 :

血栓调节蛋白

同义词 :

Thrombomodulin; TM; Fetomodulin; CD141; THBD; THRM;

分子量 :

60329分子量

Protein Function :

Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

Subcellular Localization :

Membrane; Single-pass type I membrane protein.

组织特异性 :

Endothelial cells are unique in synthesizing thrombomodulin.

研究种类 :

Cardiovascular, Stem Cells

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