产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Periostin Monoclonal Antibody
目录 :
M01378
规格 :
100微克
价格 :
299美元
克隆性 :
单克隆
宿主 :
共轭标签 :
nonconjugated
克隆名称 :
ABIF-16
反应物种 :
人类
应用 :
免疫印迹
图像
图像 1 :
武汉博士德生物工程有限公司 M01378 图像 1
Figure 1. Western blot analysis of POSTN using anti-POSTN antibody (M01378). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POSTN antigen affinity purified polyclonal antibody (Catalog # M01378) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for POSTN.
产品信息
SKU号 :
M01378
状态 :
Enabled
名称 :
Anti-Periostin Monoclonal Antibody
目录名称 :
一抗, 单克隆抗体
基因名称 :
POSTN
价格 :
299美元
price various sizes :
100ug / $299 100ug+Free HRP Secondary BA1054 / $299 100ug+Free Biotin Secondary BA1003 / $299 30ug sample size / $99
克隆性 :
单克隆
克隆编号 :
ABIF-16
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油
描述 :
Rabbit IgG Monoclonal antibody for Periostin detection. Tested positive for WB, FC in Human
short description :
Rabbit IgG Monoclonal antibody for Periostin detection. Tested positive for WB, FC in Human
规格 :
100微克
sample size available :
30ul for $99, contact us for details
UniProt数据库编号 :
Q15063
宿主 :
免疫原 :
A synthesized peptide derived from human Periostin.
形式 :
Liquid
纯化 :
亲和色谱
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
应用细节 :
免疫印迹1:500~1:2000。 FC 1:50
应用 :
免疫印迹
反应物种 :
人类
图像标记 :
Figure 1. Western blot analysis of POSTN using anti-POSTN antibody (M01378). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POSTN antigen affinity purified polyclonal antibody (Catalog # M01378) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for POSTN.
研究种类 :
Cancer, Cardiogenesis, Cardiovascular, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton / Ecm, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Structures, Tags & Cell Markers, Vascular Inflammation
更新 :
4/22/19 1:33
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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