免疫印迹, 免疫细胞化学, 流式细胞仪
图像 1 :
Western blot analysis of MUC16 expression in Human ovary cancer lysate (M01641). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC16 monoclonal antibody (Catalog # M01641) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC16
流式细胞仪, IF, 免疫细胞化学, 免疫印迹
免疫印迹1:5000-1:20000。 免疫细胞化学/IF 1:50-1:200。 流式细胞仪1:100
Boster Bio Anti-MUC16/Ca125 Rabbit Monoclonal Antibody catalog # M01641. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
A synthesized peptide derived from human MUC16
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Mucin-16 ; MUC-16; Ovarian cancer-related tumor marker CA125 ; CA-125 ; Ovarian carcinoma antigen CA125 ; MUC16 ; CA125 ;
Protein Function :
Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Subcellular Localization :
Cell membrane; Single-pass type I membrane protein. Secreted, extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
Cancer, Cell Type Markers, Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Tags & Cell Markers, Tumor Antigens, Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
Premium Provider of Antibodies and ELISA Kits