产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Cardiac FABP Fabp3 Antibody Picoband™ (monoclonal, 6C4)
目录 :
M01734
规格 :
100µg/vial
价格 :
315美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
6C4
反应物种 :
小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化
图像
图像 1 :
武汉博士德生物工程有限公司 M01734 图像 1
Figure 1. Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody (M01734). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates, . Lane 2: mouse heart tissue lysates, . Lane 3: rat heart tissue lysates, . Lane 4: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cardiac FABP antigen affinity purified monoclonal antibody (Catalog # M01734) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
图像 2 :
武汉博士德生物工程有限公司 M01734 图像 2
Figure 2. IHC analysis of Cardiac FABP using anti-Cardiac FABP antibody (M01734). Cardiac FABP was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Cardiac FABP Antibody (M01734) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
M01734
产品名称 :
Anti-Cardiac FABP Fabp3 Antibody Picoband™ (monoclonal, 6C4)
价格 :
315美元
规格 :
100µg/vial
克隆性 :
单克隆
克隆编号 :
6C4
宿主 :
小鼠
反应物种 :
小鼠, 大鼠
应用(s) :
酶联免疫吸附测定, 免疫组化, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml. ELISA (Cap), 1-5µg/ml.
描述 :
Boster Bio Anti-Cardiac FABP Fabp3 Antibody Picoband™ (monoclonal, 6C4) catalog # M01734. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
Fabp3
UniProt数据库编号 :
P07483
免疫原 :
E. coli-derived rat Cardiac FABP recombinant protein (Position: A2-A133).
形式 :
冻干
Contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
交叉活性 :
No cross reactivity with other proteins.
抗体亚型 :
IgG1
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
脂肪酸结合蛋白3
同义词 :
脂肪酸结合蛋白, 心;脂肪酸结合蛋白3;心类型脂肪酸结合蛋白;H-FABP;Fabp3
Protein Function :
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
Subcellular Localization :
细胞质
组织特异性 :
Heart, but also skeletal muscle, kidney, brain and mammary gland.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
背景 :
Heart-type fatty acid binding protein (hFABP), also known as mammary-derived growth inhibitor, is a protein that in humans is encoded by the FABP3 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer. Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP.
研究种类 :
Cardiovascular, Fatty Acid Oxidation, Fatty Acids, Lipid And Lipoprotein Metabolism, Lipids / Lipoproteins, Mesenchymal Stem Cells, Metabolic Signaling Pathways, Metabolism, Pathways And Processes, Redox Metabolism, Stem Cells
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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