Anti-Midkine MDK Rabbit Monoclonal Antibody | 武汉博士德生物工程有限公司 M01823 产品信息

产品简要

公司名称 :

武汉博士德生物工程有限公司

产品类型 :

抗体

产品名称 :

Anti-Midkine MDK Rabbit Monoclonal Antibody

M01823

规格 :

100微升

价格 :

315美元

克隆性 :

单克隆

宿主 :

domestic rabbit

共轭标签 :

未共轭

克隆名称 :

EBI-13

反应物种 :

人类

应用 :

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

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图像

图像 1 :

Western blot analysis of Midkine expression in Midkine Recombinant protein (M01823). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDK monoclonal antibody (Catalog # M01823) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDK

图像 2 :

Immunohistochemical analysis of paraffin-embedded human liver carcinoma, using Midkine Antibody (M01823). MDK was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MDK Antibody (M01823)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

产品信息

SKU号 :

M01823

产品名称 :

Anti-Midkine MDK Rabbit Monoclonal Antibody

价格 :

315美元

规格 :

100微升

克隆性 :

单克隆

克隆编号 :

EBI-13

宿主 :

兔

反应物种 :

人类

应用(s) :

免疫沉淀, IF, 免疫组化, 免疫细胞化学, 免疫印迹

应用细节 :

免疫印迹1:500-1:2000。免疫组化1:50-1:200。免疫细胞化学/IF 1:50-1:200。免疫沉淀1:30

描述 :

Boster Bio Anti-Midkine MDK Rabbit Monoclonal Antibody catalog # M01823. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human.

浓度 :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

基因名称 :

MDK

UniProt数据库编号 :

P21741

免疫原 :

A synthesized peptide derived from human Midkine

形式 :

液体

Contents :

兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA

纯化 :

亲和色谱

储存 :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

基因全称 :

Midkine

同义词 :

Midkine; MK; Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein; MDK; MK1, NEGF2;

分子量 :

15585分子量

Protein Function :

Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development (By similarity).

Subcellular Localization :

Secreted.

组织特异性 :

Expressed in various tumor cell lines. In insulinoma tissue predominantly expressed in precancerous lesions.

研究种类 :

Growth And Development, Neurogenesis, Neurology Process, Neuroscience

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公司信息