抗体

Anti-IKK alpha+beta CHUK Rabbit Monoclonal Antibody

M01918

100微升

315美元

单克隆

domestic rabbit

未共轭

FCC-3

人类, 小鼠, 大鼠

免疫印迹, 免疫细胞化学, 免疫沉淀

Anti-IKK alpha+beta CHUK Rabbit Monoclonal Antibody

315美元

100微升

单克隆

FCC-3

兔

人类, 小鼠, 大鼠

免疫沉淀, IF, 免疫细胞化学, 免疫印迹

免疫印迹1:500-1:1000。 免疫细胞化学/IF 1:50-1:100。 免疫沉淀1:50

Boster Bio Anti-IKK alpha+beta CHUK Rabbit Monoclonal Antibody catalog # M01918. Tested in IP, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

CHUK

O15111

A synthesized peptide derived from human IKK alpha+beta

液体

兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA

亲和色谱

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Inhibitor of nuclear factor kappa-B kinase subunit alpha

Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; 2.7.11.10; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16; CHUK; IKKA, TCF16;

84640分子量

Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa- B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3.

Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.

Widely expressed.

Atherosclerosis, Cardiovascular, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Nfkb Pathway, Nuclear Signaling Pathways, Protein Phosphorylation, Ser / Thr Kinases, Signal Transduction, Tlr Signaling, Vascular Inflammation