产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Cytokeratin 19 KRT19 Antibody Picoband™ (monoclonal, 3D4)
目录 :
M02101-2
规格 :
100µg/vial
价格 :
315美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
3D4
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Figure 1. Western blot analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (M02101-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human placenta tissue lysates, . Lane 3: human SK-OV-3 whole cell lysates, . Lane 4: human COLO-320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cytokeratin 19 antigen affinity purified monoclonal antibody (Catalog # M02101-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
图像 2 :
Figure 2. IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (M02101-2). Cytokeratin 19 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-Cytokeratin 19 Antibody (M02101-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
图像 3 :
Figure 3. IF analysis of Cytokeratin 19 using anti- Cytokeratin 19 antibody (M02101-2). Cytokeratin 19 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL mouse anti- Cytokeratin 19 Antibody (M02101-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:200 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
产品信息
SKU号 :
M02101-2
产品名称 :
Anti-Cytokeratin 19 KRT19 Antibody Picoband™ (monoclonal, 3D4)
价格 :
315美元
规格 :
100µg/vial
克隆性 :
单克隆
克隆编号 :
3D4
宿主 :
小鼠
反应物种 :
人类
应用(s) :
IF, 免疫组化, 免疫印迹
应用细节 :
免疫印迹, 0.1 0.5µg/毫升。 免疫组化(石蜡包埋切片), 0.5-1µg/毫升。 免疫荧光, 2µg/毫升
描述 :
Boster Bio Anti-Cytokeratin 19 KRT19 Antibody Picoband™ (monoclonal, 3D4) catalog # M02101-2. Tested in IF, IHC, WB applications. This antibody reacts with Human.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
KRT19
UniProt数据库编号 :
P08727
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human Cytokeratin 19 (334-372aa QLAHIQALISGIEAQLGDVRADSERQNQEYQRLMDIKSR), different from the related mouse and rat sequences by nine amino acids.
形式 :
冻干
Contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
交叉活性 :
No cross reactivity with other proteins.
抗体亚型 :
IgG1
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
角蛋白19
同义词 :
Keratin, type I cytoskeletal 19; Cytokeratin-19; CK-19; Keratin-19; K19; KRT19
Protein Function :
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
组织特异性 :
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
背景 :
Keratin, type I cytoskeletal 19 is a protein that in humans is encoded by the KRT19 gene. The protein encoded by this gene is a member of the keratin family. It is specifically expressed in the periderm, the transiently superficial layer that envelops the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. Due to its high sensitivity, KRT19 is the most used marker for the RT-PCR-mediated detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. Keratin 19 is often used together with keratin 8 and keratin 18 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.
研究种类 :
Class I, Cytoskeleton, Cytoskeleton / Ecm, Intermediate Filaments, Signal Transduction
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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