产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗胱氨酸-天冬氨酸蛋白酶10兔单克隆抗体
目录 :
M02190-1
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
ICH-3
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫沉淀, 流式细胞仪
图像
图像 1 :
武汉博士德生物工程有限公司 M02190-1 图像 1
Western blot analysis of Caspase-10 expression in Jurkat cell lysate (M02190-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP10 monoclonal antibody (Catalog # M02190-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP10
产品信息
SKU号 :
M02190-1
产品名称 :
抗胱氨酸-天冬氨酸蛋白酶10兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
ICH-3
宿主 :
反应物种 :
人类
应用(s) :
流式细胞仪, 免疫沉淀, 免疫组化, 免疫印迹
应用细节 :
免疫印迹1:500-1:2000。 免疫组化1:50-1:100。 免疫沉淀1:50。 流式细胞仪1:50
描述 :
Boster Bio Anti-Caspase-10 Rabbit Monoclonal Antibody catalog # M02190-1. Tested in Flow Cytometry, IP, IHC, WB applications. This antibody reacts with Human.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
CASP10
UniProt数据库编号 :
Q92851
免疫原 :
A synthesized peptide derived from human Caspase-10
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
胱氨酸-天冬氨酸蛋白酶10
同义词 :
Caspase-10; CASP-10; 3.4.22.63; Apoptotic protease Mch-4; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2; ICE-like apoptotic protease 4; Caspase-10 subunit p23/17; Caspase-10 subunit p12; CASP10; MCH4;
分子量 :
58951分子量
Protein Function :
Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp- -AMC and Asp-Glu-Val-Asp- -AMC.
组织特异性 :
Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
研究种类 :
Apoptosis, Apoptotic Markers, Cancer, Caspases Etc, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways And Processes, Proteolysis / Ubiquitin, Proteolytic Enzymes
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits