产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗JNK1/JNK3 MAPK8兔单克隆抗体
目录 :
M02608
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
免疫细胞化学-13
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫沉淀
图像
图像 1 :
武汉博士德生物工程有限公司 M02608 图像 1
Western blot analysis of JNK1/JNK3 expression in HeLa cell lysate (M02608). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK8 monoclonal antibody (Catalog # M02608) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK8
产品信息
SKU号 :
M02608
产品名称 :
抗JNK1/JNK3 MAPK8兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
免疫细胞化学-13
宿主 :
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
免疫沉淀, 免疫印迹
应用细节 :
免疫印迹1:1000-1:2000。 免疫沉淀1:50
描述 :
Boster Bio Anti-JNK1/JNK3 MAPK8 Rabbit Monoclonal Antibody catalog # M02608. Tested in IP, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
MAPK8
UniProt数据库编号 :
P45983
免疫原 :
A synthesized peptide derived from human JNK1/JNK3
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
丝裂原活化蛋白激酶8
同义词 :
丝裂原活化蛋白激酶8;地图激酶8;MAPK 8;2.7。 11.24;JNK-46;压力激活的蛋白激酶1c;SAPK1c;应激活化蛋白激酶JNK1;c Jun氨基末端激酶1;MAPK8;JNK1, PRKM8, SAPK1, SAPK1C;
分子量 :
48296分子量
Protein Function :
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692).
Subcellular Localization :
Cytoplasm. Nucleus.
研究种类 :
Cancer, Cancer Susceptibility, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Mapk Pathway, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Proto-Oncogenes, Ser / Thr Kinases, Serine/Threonine Kinases, Signal Transduction, Tlr Signaling, Transcription
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits