产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Cytochrome C CYCS Antibody Picoband™ (monoclonal, 15F10)
目录 :
M03529-5
规格 :
100µg/vial
价格 :
315美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
15F10
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
图像
图像 1 :
武汉博士德生物工程有限公司 M03529-5 图像 1
Figure 1. Flow Cytometry analysis of A431 cells using anti-Cytochrome C antibody (M03529-5). Overlay histogram showing A431 cells stained with M03529-5 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome C Antibody (M03529-5,1ug/1x10 6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
图像 2 :
武汉博士德生物工程有限公司 M03529-5 图像 2
Figure 2. Flow Cytometry analysis of A431 cells using anti-Cytochrome C antibody (M03529-5). Overlay histogram showing A431 cells stained with M03529-5 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome C Antibody (M03529-5,1ug/1x10 6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
产品信息
SKU号 :
M03529-5
产品名称 :
Anti-Cytochrome C CYCS Antibody Picoband™ (monoclonal, 15F10)
价格 :
315美元
规格 :
100µg/vial
克隆性 :
单克隆
克隆编号 :
15F10
宿主 :
小鼠
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
流式细胞仪, IF, 免疫组化-P, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹, 0.1 0.5µg/毫升。 免疫组化(石蜡包埋切片), 0.5-1µg/毫升。 免疫细胞化学/免疫荧光, 2µg/毫升, 人类。 流式细胞仪, 1-3μg/1x106细胞
描述 :
Boster Bio Anti-Cytochrome C CYCS Antibody Picoband™ (monoclonal, 15F10) catalog # M03529-5. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
CYCS
UniProt数据库编号 :
P99999
免疫原 :
E.coli-derived human Cytochrome C recombinant protein (Position: G2-E105). Human Cytochrome C shares 91% amino acid (aa) sequence identity with both mouse and rat Cytochrome C.
形式 :
冻干
Contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
交叉活性 :
No cross reactivity with other proteins.
抗体亚型 :
IgG1
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
cytochrome c, somatic
同义词 :
细胞色素C;CYCS;CYC
Protein Function :
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Subcellular Localization :
Mitochondrion intermembrane space. Loosely associated with the inner membrane.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
背景 :
CYCS is also known as CYC, HCS or THC4. This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.
研究种类 :
Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Oxidative Phosphorylation, Pathways And Processes, Signal Transduction
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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