产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Flotillin 1 FLOT1 Rabbit Monoclonal Antibody
目录 :
M04517
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
BHG-6
反应物种 :
人类, 小鼠
应用 :
免疫印迹
图像
图像 1 :
武汉博士德生物工程有限公司 M04517 图像 1
Western blot analysis of Flotillin 1 expression in HeLa cell lysate (M04517). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLOT1 monoclonal antibody (Catalog # M04517) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLOT1
产品信息
SKU号 :
M04517
产品名称 :
Anti-Flotillin 1 FLOT1 Rabbit Monoclonal Antibody
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
BHG-6
宿主 :
反应物种 :
人类, 小鼠
应用(s) :
免疫印迹
应用细节 :
免疫印迹1:500-1:2000
描述 :
Boster Bio Anti-Flotillin 1 FLOT1 Rabbit Monoclonal Antibody catalog # M04517. Tested in WB applications. This antibody reacts with Human, Mouse.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
FLOT1
UniProt数据库编号 :
O75955
免疫原 :
A synthesized peptide derived from human Flotillin 1
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
Flotillin-1
同义词 :
Flotillin-1; FLOT1;
分子量 :
47355分子量
Protein Function :
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Subcellular Localization :
Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
研究种类 :
Actin Binding Proteins, Actin Etc, Cell Biology, Cell Cycle, Cell Division, Coat Proteins, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Protein Trafficking, Signal Transduction, Vesicle Transport
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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