产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗TCF3 TCF7L1兔单克隆抗体
目录 :
M05152
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
CAH-20
反应物种 :
人类
应用 :
免疫印迹
图像
图像 1 :
武汉博士德生物工程有限公司 M05152 图像 1
Western blot analysis of TCF3 expression in A431 cell lysate (M05152). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCF7L1 monoclonal antibody (Catalog # M05152) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TCF7L1
产品信息
SKU号 :
M05152
产品名称 :
抗TCF3 TCF7L1兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
CAH-20
宿主 :
反应物种 :
人类
应用(s) :
免疫印迹
应用细节 :
免疫印迹1:500-1:2000
描述 :
Boster Bio Anti-TCF3 TCF7L1 Rabbit Monoclonal Antibody catalog # M05152. Tested in WB applications. This antibody reacts with Human.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
TCF7L1
UniProt数据库编号 :
Q9HCS4
免疫原 :
A synthesized peptide derived from human TCF3
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
Transcription factor 7-like 1
同义词 :
Transcription factor 7-like 1; HMG box transcription factor 3; TCF-3; TCF7L1; TCF3;
分子量 :
62631分子量
Protein Function :
Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis (By similarity). Down-regulates NQO1, leading to increased mitomycin c resistance.
Subcellular Localization :
Nucleus.
组织特异性 :
Detected in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium.
研究种类 :
Chromatin Binding Proteins, DNA / RNA Binding, Domain Families, Epigenetics and Nuclear Signaling, Nuclear, Signaling Pathways, Stem Cells, Transcription, Transcription Factors
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits