免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
图像 1 :
Immunohistochemical analysis of paraffin-embedded human bladder, using alpha Tubulin Antibody(M08382-1). TUBA1B was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TUBA1B Antibody (M08382-1)overnight at 4â„ƒ. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37â„ƒ. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 2 :
Western blot analysis of alpha Tubulin 4A in NIH/3T3 cell lysate (M08382-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBA1B monoclonal antibody (Catalog # M08382-1) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBA1B
人类, 小鼠, 大鼠
流式细胞仪, 免疫沉淀, IF, 免疫组化, 免疫细胞化学, 免疫印迹
免疫印迹1:5000-1:10000。 免疫组化1:50-1:200。 免疫细胞化学/IF 1:50-1:200。 免疫沉淀1:50。 流式细胞仪1:50
Boster Bio Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody catalog # M08382-1. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
A synthesized peptide derived from human alpha Tubulin
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain; TUBA1B;
Protein Function :
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Subcellular Localization :
Cytoskeleton, Cytoskeleton / Ecm, Microtubules, Signal Transduction, Tubulin
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