产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Methyl-Histone H3 (di K4) Rabbit Monoclonal Antibody
目录 :
M12477-1
规格 :
100 ug/vial
价格 :
299美元
克隆性 :
单克隆
宿主 :
共轭标签 :
nonconjugated
克隆名称 :
AFF-8
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 染色质免疫沉淀
图像
图像 1 :
武汉博士德生物工程有限公司 M12477-1 图像 1
Immunohistochemical analysis of paraffin-embedded human liver, using Methyl-Histone H3 (di K4) Antibody(M12477-1). HIST1H3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H3A Antibody (M12477-1)overnight at 4 . Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 2 :
武汉博士德生物工程有限公司 M12477-1 图像 2
Western blot analysis of Methyl-Histone H3 (di K4) expression in HeLa cell lysate (M12477-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
产品信息
SKU号 :
M12477-1
状态 :
Enabled
名称 :
Anti-Methyl-Histone H3 (di K4) Rabbit Monoclonal Antibody
目录名称 :
一抗, 单克隆抗体, 兔单克隆抗体, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
HIST1H3A
价格 :
299美元
price various sizes :
100ug / $299 100ug+Free HRP Secondary BA1054 / $299 100ug+Free Biotin Secondary BA1003 / $299 30ug sample size / $99
克隆性 :
单克隆
克隆编号 :
AFF-8
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油
描述 :
detection. Host: Rabbit.Size: 100ug/vial. Tested applications: ChIP, Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3J information: Molecular Weight: 15404 MW; Subcellular Localization: Nucleus. Chromosome.
HIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3
EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H
3J
Monoclonal antibody for
short description :
Anti-Methyl-Histone H3 (di K4) Rabbit Monoclonal Antibodytested for ChIP, Flow Cytometry, IP, IF, IHC, ICC, WB in Human, Mouse, Rat
规格 :
100 ug/vial
sample size available :
30ul for $99, contact us for details
UniProt数据库编号 :
P68431
宿主 :
免疫原 :
A synthesized peptide derived from human Methyl-Histone H3 (di K4)
形式 :
Liquid
纯化 :
亲和色谱
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
应用细节 :
免疫印迹1:500-1:2000。 免疫组化1:50-1:200。 免疫细胞化学/IF 1:50-1:200。 免疫沉淀1:50。 流式细胞仪1:50
应用 :
ChIP, 免疫沉淀, IF, 免疫组化, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Immunohistochemical analysis of paraffin-embedded human liver, using Methyl-Histone H3 (di K4) Antibody(M12477-1). HIST1H3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H3A Antibody (M12477-1)overnight at 4 . Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Western blot analysis of Methyl-Histone H3 (di K4) expression in HeLa cell lysate (M12477-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
研究种类 :
Chip'Ing Antibodies, Epigenetics And Nuclear Signaling, Histones, Unmodified
同义词 :
组蛋白H3.1;组蛋白H3/a;组蛋白H3/b;组蛋白H3/c;组蛋白H3/d;组蛋白H3/f;组蛋白H3/h;组蛋白H3/i;组蛋白H3/j;组蛋白H3/k;组蛋白H3/l;HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;
基因全称 :
组蛋白H3.1
分子量 :
15404分子量
protein function :
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
subcellular localization :
Nucleus. Chromosome.
更新 :
4/22/19 1:33
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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